Clone:
PK136
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2aκ
Applications:
FC, MICS, IF, IHC
Alternative names:
KLRB1C, CD161, Ly-59, NK-RP1, NK-1, Nk-1.2, Nkrp1c, Ly-55c

Extended validation for NK1.1 Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with PK136
REA1162++
694370+
Cells were incubated with an excess of purified unconjugated NK1.1 (PK136) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using NK1.1 (PK136). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using NK1.1 (PK136). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using NK1.1 (PK136). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for NK1.1 Antibody, anti-mouse

Overview

The PK136 monoclonal antibody reacts with NK1.1, also known as CD161. NK1.1 is expressed by natural killer cells and a subset of T cells in the mouse strains C57BL/6, FVB/N, and NZB, but not in the mouse strains AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129.

Alternative names

KLRB1C, CD161, Ly-59, NK-RP1, NK-1, Nk-1.2, Nkrp1c, Ly-55c

Detailed product information

Technical specifications

ClonePK136
Clonalitymonoclonal
Isotypemouse IgG2aκ
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Speciesmouse
AntigenNK1.1
Alternative names of antigenKLRB1C, CD161, Ly-59, NK-RP1, NK-1, Nk-1.2, Nkrp1c, Ly-55c
Molecular mass of antigen [kDa]25
Distribution of antigenT cells, NK cells
Entrez Gene ID17059
RRIDAB_2727975, AB_2727927, AB_2727573, AB_2727596, AB_2733163, AB_2733164, AB_2728038, AB_2728015, AB_2660600, AB_2660601, AB_2727578

Resources for NK1.1 Antibody, anti-mouse

Certificates

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