Recombinant mouse M-CSF regulates the differentiation, proliferation, and survival of osteoclasts, macrophages, monocytes, and their hematopoietic progenitors. As a potent hematopoietic cytokine, macrophage colony-stimulating factor (M-CSF) is involved in diverse processes, such as regulation of atherosclerosis or brain, bone resorption, placental development, and inflammatory responses. This recombinant protein has been optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Mouse M-CSF

Figures

Figure 1

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Mouse M-CSF activity assay.
The biological activity of Mouse M-CSF, premium grade, was determined by proliferation assay using M-NFS-60 cells. Activity of Mouse M-CSF, premium grade, (red line) was compared to another commercially available product (black line).

Figure 1

Mouse M-CSF activity assay.
The biological activity of Mouse M-CSF, premium grade, was determined by proliferation assay using M-NFS-60 cells. Activity of Mouse M-CSF, premium grade, (red line) was compared to another commercially available product (black line).

Figure 2

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SDS-PAGE of Mouse M-CSF, premium grade under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Mouse M-CSF, premium grade under reduced (R) and non-reduced (NR) conditions.

Figure 3

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Mass spectrometry analysis (ESI-MS) of Mouse M-CSF, premium grade. The peak corresponds to the calculated molecular mass of 36141 Da.

Figure 3

Mass spectrometry analysis (ESI-MS) of Mouse M-CSF, premium grade. The peak corresponds to the calculated molecular mass of 36141 Da.

Specifications for Mouse M-CSF

Overview

Recombinant mouse M-CSF regulates the differentiation, proliferation, and survival of osteoclasts, macrophages, monocytes, and their hematopoietic progenitors. As a potent hematopoietic cytokine, macrophage colony-stimulating factor (M-CSF) is involved in diverse processes, such as regulation of atherosclerosis or brain, bone resorption, placental development, and inflammatory responses. This recombinant protein has been optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse M-CSF can be used for a variety of applications, including:
  • Generation of bone marrow-derived macrophages.
  • Differentiation of osteoclasts.
  • Proliferation and apoptosis assays.

Detailed product information

Background information

Macrophage-colony stimulating factor (M-CSF), a four α-helical bundle cytokine, is a potent hematopoietic regulator. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.

Biological activity

  • Proliferation of M-NFS-60 cells
  • research grade: ≥ 1×
    10
    5
    U/mg
  • premium grade: ≥ 2×
    10
    5
    U/mg
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Mouse M-CSF

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Mouse M-CSF

Publications

  1. Tanne, A. et al. (2009)
    A murine DC-SIGN homologue contributes to early host defense against
    Mycobacterium tuberculosis
    .
    J. Exp. Med. 206(10): 2205-2220
  2. Herold, J. et al. (2014) Tetanus toxoid-pulsed monocyte vaccination for augmentation of collateral vessel growth. J Am Heart Assoc. 3(2): e000611
  3. Guery L. et al. (2014) Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118: 4694-4704

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