The Mouse IL-5 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of murine IL-5-secreting cells.

Data and images for Mouse IL-5 Secretion Assay – Cell Enrichment and Detection Kit (PE)

Figures

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with keyhole limpet hemocyanin (KLH) as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-5 using the Mouse IL-5 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 233 IL-5-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only six IL-5-secreting CD4
+
T cells were detected per 10
6
CD4
+
T cells after enrichment.
Stimulated sample
Before enrichment
After enrichment
View details

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with keyhole limpet hemocyanin (KLH) as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-5 using the Mouse IL-5 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 233 IL-5-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only six IL-5-secreting CD4
+
T cells were detected per 10
6
CD4
+
T cells after enrichment.
View details

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with keyhole limpet hemocyanin (KLH) as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-5 using the Mouse IL-5 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 233 IL-5-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only six IL-5-secreting CD4
+
T cells were detected per 10
6
CD4
+
T cells after enrichment.
Unstimulated control
Before enrichment
After enrichment
View details

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with keyhole limpet hemocyanin (KLH) as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-5 using the Mouse IL-5 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 233 IL-5-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only six IL-5-secreting CD4
+
T cells were detected per 10
6
CD4
+
T cells after enrichment.
View details

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with keyhole limpet hemocyanin (KLH) as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-5 using the Mouse IL-5 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 233 IL-5-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only six IL-5-secreting CD4
+
T cells were detected per 10
6
CD4
+
T cells after enrichment.

Specifications for Mouse IL-5 Secretion Assay – Cell Enrichment and Detection Kit (PE)

Overview

The Mouse IL-5 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of murine IL-5-secreting cells.

Detailed product information

Background information

Interleukin-5 (IL-5) is predominantly secreted by CD4
+
T cells. It promotes growth and activation of eosinophils, is involved in a range of allergic reactions, and mediates immune reactions against parasites. IL-5 also acts on other cell types such as B cells.

Downstream applications

The Mouse IL-5 Secretion Assays can be used to detect and isolate murine antigen-specific, IL-5-secreting T cells after stimulation with antigen (fig. 1). The assays allow studies on T
H
2 development and commitment as well as on the mechanism of IL-5 expression.

Columns

MS, LS, or autoMACS
®
Columns.

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Mouse IL-5 Secretion Assay – Cell Enrichment and Detection Kit (PE)

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