The IL-10 Secretion Assay was developed for the sensitive detection of mouse IL-10-secreting cells.

Data and images for Mouse IL-10 Secretion Assay – Detection Kits

Figures

Figure 1

BALB/c mice were immunized i.p. with KLH (keyhole limpet hemocyanin) in incomplete Freund's adjuvant and pertussis toxin. On day 14 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IL-10 Secretion Assay - Detection Kit (APC) and Mouse IL-2 Secretion Assay - Detection Kit (PE). The plots show IL-10- and IL-2-secreting cells gated on viable CD4
+
T cells for the stimulated sample as well as for the unstimulated control.
A:
B:
Stimulated sample
Unstimulated control
View details

Figure 1

BALB/c mice were immunized i.p. with KLH (keyhole limpet hemocyanin) in incomplete Freund's adjuvant and pertussis toxin. On day 14 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IL-10 Secretion Assay - Detection Kit (APC) and Mouse IL-2 Secretion Assay - Detection Kit (PE). The plots show IL-10- and IL-2-secreting cells gated on viable CD4
+
T cells for the stimulated sample as well as for the unstimulated control.
View details

Figure 1

BALB/c mice were immunized i.p. with KLH (keyhole limpet hemocyanin) in incomplete Freund's adjuvant and pertussis toxin. On day 14 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IL-10 Secretion Assay - Detection Kit (APC) and Mouse IL-2 Secretion Assay - Detection Kit (PE). The plots show IL-10- and IL-2-secreting cells gated on viable CD4
+
T cells for the stimulated sample as well as for the unstimulated control.

Specifications for Mouse IL-10 Secretion Assay – Detection Kits

Overview

The IL-10 Secretion Assay was developed for the sensitive detection of mouse IL-10-secreting cells.

Detailed product information

Background information

Interleukin-10 (IL-10) is predominantly secreted by CD4
+
T cells and antigen-presenting cells, such as monocytes/macrophages and dendritic cells. IL-10 has important suppressive functions on immune responses and is involved in the maintenance of tolerance. IL-10 blocks activation of cytokine synthesis by T
H
1 cells, activated monocytes, and NK cells. It can stimulate the immunoglobulin production by B cells.

Downstream applications

The Mouse IL-10 Secretion Assay was used for the analysis of the sequential production of cytokines by T helper lymphocytes.
1
The assay was also used for analysis of IL-10 expression by T
H
2 cells
2
, by dendritic cells from mouse spleen
3
, and by T cells from bronchioalveolar lavages
4
.

Resources for Mouse IL-10 Secretion Assay – Detection Kits

References for Mouse IL-10 Secretion Assay – Detection Kits

Publications

  1. Donnelly, S. et al. (2014) Secreted proteins from the helminth Fasciola hepatica inhibit the initiation of autoreactive T cell responses and prevent diabetes in the NOD mouse. PLoS One 9(1)
  2. Topp, M. S. et al. (2014) Distinct and complementary roles for Aspergillus fumigatus-specific Tr1 and Foxp3+ regulatory T cells in humans and mice. Immunol. Cell Biol. 92(8): 659-670
  3. Assenmacher et al. (1998) Sequential production of IL-2, IFN-gamma and IL-10 by individual staphylococcal enterotoxin B-activated T helper lymphocytes. Eur. J. Immunol. 28: 1534-1543
  4. Löhning, M. et al. (2003) Establishment of memory for IL-10 expression in developing T helper 2 cells requires repetitive IL-4 costimulation and does not impair proliferation. Proc. Natl. Acad. Sci. U.S.A. 100: 12307-12312
  5. Edwards, A. D. et al. (2002) Microbial recognition via Toll-like receptor-dependent and -independent pathways determines the cytokine response of murine dendritic cell subsets to CD40 triggering. J. Immunol. 169: 3652-3660
  6. Mc Allister, F. et al. (2004)
    T cytotoxic-1 CD8
    +
    T cells are effector cells against pneumocystis in mice.
    J. Immunol. 172: 1132-1138

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