Recombinant mouse GM-CSF can regulate growth and differentiation of cells of the myeloid lineage such as DCs, granulocytes, monocytes, and macrophages. As a crucial part of the immune/inflammatory path, the hematopoietic cytokine serves as both an activation signal for mature myeloid cells and survival. The recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) is developed for use in functional assays, differentiation studies, and cell culture.

Data and images for Mouse GM-CSF

Figures

Figure 1

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SDS-PAGE of Mouse GM-CSF, premium grade
under reducing (R) and non-reducing (NR) conditions.

Figure 1

SDS-PAGE of Mouse GM-CSF, premium grade
under reducing (R) and non-reducing (NR) conditions.

Figure 2

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Mass spectrometry analysis (ESI-MS) of Mouse GM-CSF premium grade. The peak corresponds to the calculated molecular mass of 14108 kDa.

Figure 2

Mass spectrometry analysis (ESI-MS) of Mouse GM-CSF premium grade. The peak corresponds to the calculated molecular mass of 14108 kDa.

Specifications for Mouse GM-CSF

Overview

Recombinant mouse GM-CSF can regulate growth and differentiation of cells of the myeloid lineage such as DCs, granulocytes, monocytes, and macrophages. As a crucial part of the immune/inflammatory path, the hematopoietic cytokine serves as both an activation signal for mature myeloid cells and survival. The recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) is developed for use in functional assays, differentiation studies, and cell culture.

Applications

Mouse GM-CSF can be used for a variety of applications, including:
  • Induction of colony formation of granulocyte/macrophage progenitors in semi-solid medium.
  • In vitro generation of DCs from bone marrow or maturation of CD11c+ splenocytes.
  • Generation of antigen-presenting (DC-like) cells in primary brain cell culture.

Detailed product information

Background information

GM-CSF is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF strongly chemoattracts neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.

Biological activity

  • Proliferation of FDC-P1 cells (NIBSC 91/658)
  • research grade: ≥ 1×
    10
    7
    U/mg
  • premium grade: ≥ 5×
    10
    7
    U/mg
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Mouse GM-CSF

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Mouse GM-CSF

Publications

  1. Ait-Oufella et al. (2010) B cell depletion reduces the development of atherosclerosis in mice. J. Exp. Med. 207: 1579-1587
  2. DeLamarter, J.F. et al. (1985)
    Recombinant murine GM-CSF from
    E. coli
    has biological activity and is neutralized by a specific antiserum.
    EMBO J. 4: 2575-2581
  3. Cantini, G. et al. (2012) Immunotherapy against the radial glia marker GLAST effectively triggers specific antitumor effectors without autoimmunity. Oncoimmunology 1(6): 884-893

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