The MACSPlex Exosome Kits allow detection of 37 exosomal surface epitopes plus two isotype controls. The MACSPlex Exosome Kits comprise a cocktail of various fluorescently labeled bead populations, each coated with a specific antibody binding the respective surface epitope.The 39 bead populations can be distinguished by different fluorescence intensities by flow cytometry.

Data and images for MACSPlex Exosome Kit, human

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The MACSPlex Exosome Kit has been developed for the simultaneous flow cytometric detection of 37 surface epitopes that are known to be present on different exosomes plus two isotype control beads.
The MACSPlex Exosome Kit has been developed for the simultaneous flow cytometric detection of 37 surface epitopes that are known to be present on different exosomes plus two isotype control beads.

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Specifications for MACSPlex Exosome Kit, human

Overview

The MACSPlex Exosome Kits allow detection of 37 exosomal surface epitopes plus two isotype controls. The MACSPlex Exosome Kits comprise a cocktail of various fluorescently labeled bead populations, each coated with a specific antibody binding the respective surface epitope.The 39 bead populations can be distinguished by different fluorescence intensities by flow cytometry.

Detailed product information

Exosome are incubated with the antibody-coated MACSPlex Exosome Capture Beads. Subsequently or in parallel, exosomes bound to the MACSPlex Exosome Capture Beads are labeled with the MACSPlex Exosome Detection Reagents. The MACSPlex Exosome Detection Reagents can also be combined to create a cocktail comprising of MACSPlex Exosome Detection Reagent for CD9, CD63, and CD81. Consequently, sandwich complexes are formed between the MACSPlex Exosome Capture Bead, exosome, and the detection reagent (see Data and Images, Figure 1). These complexes can be analyzed based on the fluorescence characteristics of both the MACSPlex Exosome Capture Bead and the detection reagent. Positive signals indicate the presence of the respective surface epitope within the exosome population (see Data and Images, Figure 2). It is also possible to compare different exosome samples using the MACSPlex Exosome Kits allowing semi-quantitative analysis of differential surface epitopes.

Applications

The MACSPlex Exosome Kits allows for qualitative and semiquantitative analysis of 37 exosomal surface epitopes. The MACSPlex Exosome Kit has been developed for the simultaneous flow cytometric detection of 37 surface epitopes that are known to be present on different exosomes plus two isotype control beads.

References for MACSPlex Exosome Kit, human

Publications

  1. Martin-Jaular, L. et al. (2021) Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV‐1 infection EMBO J. 40(8): e105492
  2. Bruno, S. et al. (2020) HLSC-Derived Extracellular Vesicles Attenuate Liver Fibrosis and Inflammation in a Murine Model of Non-alcoholic Steatohepatitis Mol. Ther. 28(2): 479-489
  3. Andaloussi, S. et al. (2017) Reproducible and scalable purification of extracellular vesicles using combined bind-elute and size exclusion chromatography. Sci Rep 7(1): 11561
  4. Koliha, N. et al. (2016) Melanoma affects the composition of blood cell-derived extracellular vesicles. Front Immunol 7: 282
  5. Wiklander, O.P.B. et al. (2018) Systematic Methodological Evaluation of a Multiplex Bead-Based Flow Cytometry Assay for Detection of Extracellular Vesicle Surface Signatures. Front Immunol 9: 1326
  6. Koliha, N. et al. (2016) A novel multiplex bead-based platform highlights the diversity of extracellular vesicles. J Extracell Vesicles 5: 29975
  7. Martire, S. et al. (2021) A First Phenotypic and Functional Characterization of Placental Extracellular Vesicles from Women with Multiple Sclerosis Int J Mol Sci 22(6): 2875
  8. Pomatto, M. et al. (2021) Differential Therapeutic Effect of Extracellular Vesicles Derived by Bone Marrow and Adipose Mesenchymal Stem Cells on Wound Healing of Diabetic Ulcers and Correlation to Their Cargoes Int J Mol Sci 22(8): 3851
  9. Kaspi, H. et al. (2021)
    MSC-NTF (NurOwn
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    ) exosomes: a novel therapeutic modality in the mouse LPS-induced ARDS model
    Stem Cell Res. Ther. 12: 72
  10. Brahmer, A. et al. (2019) Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation. J. Extracell. Vesicles 8((1))
  11. Görgens, A. et al. (2019) Optimisation of imaging flow cytometry for the analysis of single extracellular vesicles by using fluorescence-tagged vesicles as biological reference material J. Extracell. Vesicles 8(1): 1587567
  12. Gupta, D. et al. (2020) Quantification of extracellular vesicles in vitro and in vivo using sensitive bioluminescence imaging J. Extracell. Vesicles 9(1)
  13. Calleri, A. et al. (2021) Protective Effects of Human Liver Stem Cell-Derived Extracellular Vesicles in a Mouse Model of Hepatic Ischemia-Reperfusion Injury Stem Cell Rev Rep. 17(2): 459
  14. Forte, D. et al. (2021)
    Distinct profile of CD34
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    cells and plasma-derived extracellular vesicles from triple-negative patients with Myelofibrosis reveals potential markers of aggressive disease
    J Exp Clin Cancer Res. 40: 49
  15. Collino, F. et al. (2020) Extracellular Vesicles Derived from Induced Pluripotent Stem Cells Promote Renoprotection in Acute Kidney Injury Model Cells 9(2): 453
  16. Shin, K-O. et al. (2020) Exosomes from Human Adipose Tissue-Derived Mesenchymal Stem Cells Promote Epidermal Barrier Repair by Inducing de Novo Synthesis of Ceramides in Atopic Dermatitis Cells 9(3): 680
  17. Vacchi, E. et al. (2020) Immune profiling of plasma-derived extracellular vesicles identifies Parkinson disease Neurol Neuroimmunol Neuroinflamm. 7(6): e866
  18. Salvi, S. et al. (2021) Detection and Investigation of Extracellular Vesicles in Serum and Urine Supernatant of Prostate Cancer Patients Diagnostics 11(3): 466

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