REAlease® CD53 Complexes, human

REAlease
®
CD53 Complexes
, human

Clone: REAL144 | Dilution: 1:50
Clone REAL144 is an antibody fragment derived from the full CD53 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL144 recognizes the CD53 antigen, a 35–42 kDa type II transmembrane glycoprotein which is also known as tetraspanin-25. CD53 is expressed on all leukocytes, including plasma cells, but not on platelets, erythrocytes, and non-hematopoietic tissues. CD53 mediates oxidative burst in monocytes and has been shown to interact with a number of proteins including interleukin 4 (IL-4), CD20, CD2, CD9, IL-2, VLA-4, and CD4. Cross-linking of CD53 on monocytes induces a strong increase in intracellular calcium ions, whereas a modest increase is seen in B lymphocytes. In resting B cells, the molecule transmits a co-stimulatory signal and promotes activation through the Ig receptor.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

MOX44, TSPAN25

Technical specifications

  • Antigen: CD53
  • Clone: REAL144
  • Isotype control: REAL Control
  • Alternative names of antigen: MOX44, TSPAN25
  • Distribution of antigen: leukocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC
  • Selected references

    1. Angelisova, P. et al. (1990) The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens. Immunogenetics 32(4): 281-285
    2. Maecker, H. T, et al. (1997) The tetraspanin superfamily: molecular facilitators. FASEB J. 11: 428-442
    3. Olweus, J. et al. (1993) CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J. Immunol. 151: 707-716
Product options: 3
for 30
tests
(1)
EUR 145,00 
for 100
tests
(1)
EUR 345,00 
for 100
tests
(1)
EUR 405,00 

Related products

3 products available