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Mouse thymocytes from 6-week-old BALB/c mice were stained with CD279 (PD1) antibodies as well as with CD3ε, CD8a, and CD4 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cells labeled with CD279 (PD1)-Biotin were stained with Anti-Biotin-PE in addition. Dot plots shown are gated on CD3ε+
cells. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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