Anti-MEK1 pS298 antibodies, human and mouse

Anti-MEK1 pS298 antibodies, human and mouse

Clone: REA375 | Dilution: 1:11
Clone REA375 recognizes the human and mouse MEK1 antigen phosphorylated at serine 298 (pS298). The dual specificity kinases MEK1 and MEK2 are activated by RAF and mediate phosphorylation of ERK1 and ERK2. MEK1 and MEK2 are very similar but differ structurally in a proline-rich domain in the C-terminal half of the catalytic core. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival, and differentiation, predominantly through the regulation of transcription, metabolism, and cytoskeletal rearrangements. MEK1 is phosphorylated at S298 by PAK. MAPK1/ERK2 phosphorylation of T292 occurs in response to cellular adhesion and leads to inhibition of S298 phosphorylation by PAK.
Additional information: Clone REA375 displays negligible binding to Fc receptors.

Alternative names

MAP2K1, CFC3, MAPKK 1, MKK1, PRKMK1, MEK1

Technical specifications

  • Antigen: MEK1 pS298
  • Clone: REA375
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: MAP2K1, CFC3, MAPKK 1, MKK1, PRKMK1, MEK1
  • Entrez Gene ID: 5604
  • Molecular mass of antigen [kDa]: 43
  • Distribution of antigen: other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC
  • Selected references

    1. Zheng, C. F. et al. (1994) Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues. EMBO J. 13(5): 1123-1131
    2. Zheng, C. F. et al. (1993) Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J. Biol. Chem. 268(15): 11435-11439
    3. Coles, L. C. et al. (2002) PAK1 primes MEK1 for phosphorylation by Raf-1 kinase during cross-cascade activation of the ERK pathway. Oncogene 21(14): 2236-2244
Product options: 4
for 30
tests
(1)
EUR 115,00 
for 100
tests
(1)
EUR 285,00 
for 30
tests
(1)
EUR 115,00 
for 100
tests
(1)
EUR 285,00 

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