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regulatory T cells were isolated from human PBMCs. The cells were fluorescently stained as indicated and analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to CD4 expression except for the CD25-APC vs. CD4 (VIT4)-VioBlue dot plot.
PBMCs before separation
Pre-enriched CD4+ cells
Isolated CD4+ CD25+ regulatory T cells
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