Anti-ACSA-2 MicroBead Kit, mouse

Anti-ACSA-2 MicroBead Kit, mouse

Anti-ACSA-2 MicroBeads (ACSA-2: astrocyte cell surface antigen-2) have been developed for the isolation or depletion of primary mouse astrocytes based on the expression of the ACSA-2 antigen. The protocol can be performed within only one hour.

Background information

The ACSA-2 antigen is specifically expressed on GLAST (ACSA-1) positive astrocytes and is therefore an exclusive marker of astrocytes in the developing and neonatal mouse central nervous system (CNS). The Anti-ACSA-2 MicroBeads allow also for purification of astrocytes that show weak GLAST (ACSA-1) expression.
The percentage of ACSA-2 positive astrocytes differs according to the mouse age and the brain region used for the cell isolation.
The isolation of primary astrocytes was tested particularly on dissociated postnatal CD-1 mouse brain tissue derived from animals younger than postnatal day 8 (P8). The protocol can be performed in only one hour. The astrocytes show excellent morphology in culture using MACS® Neuro Medium, MACS® NeuroBrew-21 with P/S and L-glutamine.
Video

How to isolate pure viable astrocytes

Watch this hands-on protocol for the isolation of astrocytes from mouse brain tissue. The Miltenyi Biotec protocol yields highly purified cells with preserved vitality and can be finished within half a day.

Product options: 2

Product information

Size
Order no.
Price

Anti-ACSA-2 MicroBead Kit, mouse - small size

  • For research use only
for 5×
10
7
total cells
130-097-679
EUR 63,00 

Anti-ACSA-2 MicroBead Kit, mouse

  • For research use only
for 1×
10
9
total cells
130-097-678
EUR 604,00