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cells were isolated from human peripheral blood mononuclear cells (PBMCs) by using the Classical Monocyte Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD14-FITC and CD16-APC and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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