CD207 (Langerin) MicroBeads, human

CD207 (Langerin) MicroBeads, human

CD207 (Langerin) MicroBeads were developed for the isolation of CD207
dendritic cells from single-cell suspensions of human epidermis.

Background information

Langerin (CD207 antigen), a 40 kDa, glycosylated type II transmembrane C-type lectin receptor, is described as the state-ofthe-art marker for identifying Langerhans cells (LCs) in humans and mice
. LCs are a subset of dendritic cells (DCs) cells that reside in epithelia; the best studied example of these antigen-presenting cells are LCs of the epidermis. Their unique identifying feature are organelles called Birbeck Granules (BGs), which can be detected by electron microscopy. CD207 is expressed at both the surface membrane and in the cytoplasmatic BGs. CD207 plays a role as an endocytic receptor. It is directly involved in antigen capture and its endocytosis and induces the formation of BGs in immature DCs

Detailed separation procedure

Single-cell suspensions of human epidermis are magnetically labeled with CD207 (Langerin) MicroBeads. The cell suspension is loaded onto a MACS
Column, in the magnetic field of a MACS Separator. The magnetically labeled cells are retained within the column. Unlabeled cells, depleted of CD207
cells, can be collected in the flow through. After removing the column from the magnetic field, CD207
cells can be eluted as the positively selected cell fraction.


MS, LS, or autoMACS
  • Selected references

    1. Valladeau, J. et al. (2000) Langerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules. Immunity 12(1): 71-81
    2. Romani, N. et al. (2010) Langerhans cells and more: langerin-expressing dendritic cell subsets in the skin. Immunol. Rev. 234(1): 120-141
Product options: 1
for 1×
total cells
EUR 610,00