Mouse IFN-α

Mouse IFN-α

IFN-α stands for interferon alpha. Mouse IFN-α is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IFN-α can be used for a variety of applications including:
  • In vitr o investigation of antitumor effects, such as antiproliferative capacities, on tumor cell lines.
  • Studies on signal transduction and gene expression on cell lines.
  • In vivo investigation of anti-tumor effects in mouse models of disease.
  • Studies of transgenic mice harboring the Cre-recombinase transgene driven by the interferon-inducible Mx promoter (Mx-Cre mice).

Background information

Type I interferons (IFNs), including IFN-α, are a family of cytokines that exert multiple functions in the immune system. The most prominent effect of IFN-α is its antiviral activity. Upon viral infections, host cells release IFN-α, which can act in an autocrine or paracrine manner to activate intracellular antiviral defense mechanisms and restrict viral replication. Furthermore, IFN-α affects the generation and function of various dendritic cell populations. Immunomodulatory activity and antitumor effects have been described both
in vivo
and
in vitro
.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Inhibition of BW cells
  • research grade: ≥ 5×
    10
    7
    IU/mL
  • Selected references

    1. Pestka, S. et al. (2004) Interferons, interferon-like cytokines, and their receptors. Immunol. Rev. 202: 8-32
    2. Takaoka, A. and Yanai, H. (2006) Interferon signalling network in innate defence. Cell. Microbiol. 8: 907-922
    3. Darnell, J. E. Jr. et al. (1994) Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science 264: 1415-1421
    4. van Boxel-Dezaire, A. H. et al. (2006) Complex modulation of cell type-specific signaling in response to type I interferons. Immunity 25(3): 361-372
    5. Kühn, R. et al. (1995) Inducible gene targeting in mice. Science 269: 1427-1429
    6. Irla, M. et al. (2010) MHC class II-restricted antigen presentation by plasmacytoid dendritic cells inhibits T cell-mediated autoimmunity. J. Exp. Med. 207(9): 1891-1905
    7. Zoglmeier, C. et al. (2011) CpG blocks immunosuppression by myeloid-derived suppressor cells in tumor-bearing mice. Clin. Cancer Res. 17(7): 1765-1775
    8. Baccala, R. et al. (2012) Anti-IFN-α/β receptor antibody treatment ameliorates disease in lupus-predisposed mice. J. Immunol. 189(12): 5976-5984
    9. Goritzka, M. et al. (2015) Alveolar macrophage-derived type I interferons orchestrate innate immunity to RSV through recruitment of antiviral monocytes. J. Exp. Med. 212(5): 699-714
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 2
HEK293 cells
200 µL
EUR 310,00 
HEK293 cells
1 mL
EUR 1 055,00