Human IFN-γ1b

Human IFN-γ1b

IFN-γ stands for interferon γ. Human IFN-γ is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IFN-γ1b can be used for a variety of applications, including:
  • Stimulation of monocytes, macrophages, and microglia.
  • In vitro maturation of Mo-DCs.
  • Apoptosis and cell growth inhibition assays.
  • Adhesion and migration assays.

Alternative names

IFN-γ

Background information

IFN-γ, the only type II interferon, possesses potent anti-viral activity and immunoregulatory properties. It is structurally unrelated to the type I interferons and binds to a different receptor expressed by most immune cells. IFN-γ is produced predominantly by NK cells and T cells. IFN-γ enhances NK cell and macrophage activity and regulates B cell functions, like immunglobulin production and class switching. In addition, IFN-γ orchestrates leukocyte attraction and directs growth, maturation, and differentiation of many cell types. The activity of human IFN-γ is species-specific.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of HT-29 cells (NIAID Gxg01-902-535)
  • premium grade: ≥ 2×
    10
    7
    IU/mg
    (typical activity: ≥ 5×
    10
    7
    IU/mg
    )
  • research grade: ≥ 1×
    10
    7
    IU/mg
  • Selected references

    1. Wong, V.L. et al. (1989) Growth-inhibitory activity of interferon-beta against human colorectal carcinoma cell lines. Int. J. Cancer 43: 526-530
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
E. coli
10 µg
EUR 55,00 
E. coli
25 µg
EUR 80,00 
E. coli
10 µg
EUR 70,00 
E. coli
25 µg
EUR 100,00 
E. coli
100 µg
EUR 210,00 
E. coli
1000 µg
EUR 1 050,00