The Large Scale IFN-γ Secretion Assay - Enrichment Kit is suitable for the enrichment of human IFN-γ-secreting, antigen-specific T cells from leukapheresis harvests, PBMCs, or other leukocyte-containing single-cell preparations. Different from the standard Cytokine Secretion Assays, this assay uses a direct strategy for the magnetic labeling of the target cells.

Data and images for Large Scale IFN-γ Secretion Assay – Enrichment Kit, human

Figures

Figure

View details
Principle of the MACS
®
Cytokine Secretion Assay Technology using direct magnetic labeling of the target cells.
Principle of the MACS
®
Cytokine Secretion Assay Technology using direct magnetic labeling of the target cells.

Specifications for Large Scale IFN-γ Secretion Assay – Enrichment Kit, human

Overview

The Large Scale IFN-γ Secretion Assay - Enrichment Kit is suitable for the enrichment of human IFN-γ-secreting, antigen-specific T cells from leukapheresis harvests, PBMCs, or other leukocyte-containing single-cell preparations. Different from the standard Cytokine Secretion Assays, this assay uses a direct strategy for the magnetic labeling of the target cells.

Detailed product information

Background information

The Large Scale IFN-γ Secretion Assay Enrichment Kit is exclusively designed for research use. Nevertheless, it can be used in combination with the manual cell separation systems as well as the autoMACS® Separator, the autoMACS® Pro Separator, and the CliniMACS® Plus Instrument.The MicroBeads are directly conjugated to a secondary IFN-γ-specific antibody (see figure 1). The kit includes Anti-IFN-γ-PE staining antibodies for flow cytometric analysis.

Applications

The Large Scale IFN-γ Secretion Assay - Enrichment Kit allows the concomitant enrichment of viable CD4- and CD8-positive IFN-γ secreting T cells, e.g., for the large-scale generation of antigen-specific cell lines or clones. Tedious subcloning is not necessary and both CD4
+
and CD8
+
T cell lines may easily be expanded.

References for Large Scale IFN-γ Secretion Assay – Enrichment Kit, human

Publications

  1. Campbell J.D. (2003) Detection and enrichment of antigen-specific CD4+ and CD8+ T cells based on cytokine secretion. Methods 31(2): 150-159
  2. Feuchtinger T. et al. (2004)
    Isolation and expansion of human adenovirus-specific CD4
    +
    and CD8
    +
    T cells according to IFN-gamma secretion for adjuvant immunotherapy.
    Exp. Hematol. 32: 282-289
  3. Rauser G. et al. (2004)
    Rapid generation of combined CMV-specific CD4
    +
    and CD8
    +
    T-cell lines for adoptive transfer into recipients of allogeneic stem cell transplants.
    Blood 103(9): 3565-3572
  4. Feuchtinger, T. et al. (2006) Safe adoptive transfer of virus-specific T-cell immunity for the treatment of systemic adenovirus infection after allogeneic stem cell transplantation. Br J Haematol 134(1): 64-76

Related products for
Large Scale IFN-γ Secretion Assay – Enrichment Kit, human

4 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?