Recombinant human IL-15 (interleukin 15) promotes proliferation of activated T, NK, and B cells . The regulatory cytokine IL-15 has important diverse roles in the immune system. It is structurally related to IL-2 and they share many functional characteristics. The recombinant protein is optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Human IL-15

Figures

Figure 1

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SDS-PAGE of Human IL-15, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 1

SDS-PAGE of Human IL-15, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

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Mass spectrometry analysis (ESI-MS) of Human IL-15, premium grade. The peak corresponds to the calculated molecular mass of 12770 Da.

Figure 2

Mass spectrometry analysis (ESI-MS) of Human IL-15, premium grade. The peak corresponds to the calculated molecular mass of 12770 Da.

Specifications for Human IL-15

Overview

Recombinant human IL-15 (interleukin 15) promotes proliferation of activated T, NK, and B cells . The regulatory cytokine IL-15 has important diverse roles in the immune system. It is structurally related to IL-2 and they share many functional characteristics. The recombinant protein is optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-15 can be used for a variety of applications, including:
  • Activation and expansion of NK and NKT cells.
  • In vitro differentiation of NK cells, e.g., from purified CD34+ cells.
  • In vitro T cell expansion, e.g., of naive CD8+ T cells, and T cell activation, e.g., of CTLs.

Detailed product information

Background information

IL-15 is a member of the four α-helix bundle cytokine family. It is produced by different cell types, including epithelial cells, monocytes, muscle and placenta cells. IL-15 is a potent lymphoid cell growth factor. It stimulates the proliferation of activated T cells and promotes the generation of cytotoxic T lymphocytes (CTLs). IL-15 also induces the generation, proliferation, and activation of NK cells as well as B cell growth and immunoglobulin production. In addition, IL-15 is important for the maintenance of CD8
+
memory T cells. For binding and signaling IL-15 uses the unique IL-15 receptor α-chain, but shares the β- and γ-chain of the IL-2 receptor.

Biological activity

  • Proliferation of CTLL-2 cells (NIBSC 95/554)
  • premium grade: ≥ 5×
    10
    6
    U/mg
    (typical activity: ≥ 1.1×
    10
    7
    U/mg
    )
  • research grade: ≥ 2×
    10
    6
    U/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human IL-15

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human IL-15

Publications

  1. Soman, G. et al. (2009) MTS dye based colorimetric CTLL-2 cell proliferation assay for product release and stability monitoring of interleukin-15: assay qualification, standardization and statistical analysis. J. Immunol. Methods 348: 83-94
  2. Dietz, L. et al. (2010) Tracking human contact allergens: from mass spectrometric identification of peptide-bound reactive small chemicals to chemical-specific naive human T-cell priming. Toxicol. Sci. 117(2): 336-347
  3. Fuchs, S. et al. (2014)
    Patients with T
    +/low
    NK
    +
    IL-2 receptor γ chain deficiency have differentially-impaired cytokine signaling resulting in severe combined immunodeficiency.
    Eur. J. Immunol. 44(10): 3129-3140
  4. Alvarez-Breckenridge, C. A. et al. (2012) The histone deacetylase inhibitor valproic acid lessens NK cell action against oncolytic virus-infected glioblastoma cells by inhibition of STAT5/T-BET signaling and generation of gamma interferon. J. Virol. 86(8): 4566-4577
  5. Gordy, L. E. et al. (2011) IL-15 regulates homeostasis and terminal maturation of NKT cells. J. Immunol. 187(12): 6335-6345
  6. Juelke, K. et al. (2010)
    CD62L expression identifies a unique subset of polyfunctional CD56
    dim
    NK cells.
    Blood 116(8): 1299-1307

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