Alternative names:
HBGF-4

Data and images for Human FGF-4

Figures

Figure 1

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Human FGF-4 activity assay.
The biological activity of Human FGF-4, premium grade was determined by proliferation assay in the presence of sodium heparin using mouse 3T3 cells. Activity of Human FGF-4, premium grade, (red line) was compared to another commercially available product (black line).

Figure 1

Human FGF-4 activity assay.
The biological activity of Human FGF-4, premium grade was determined by proliferation assay in the presence of sodium heparin using mouse 3T3 cells. Activity of Human FGF-4, premium grade, (red line) was compared to another commercially available product (black line).

Figure 2

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Human FGF-4 biological activity.
Activity of Human FGF-4 premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 2

Human FGF-4 biological activity.
Activity of Human FGF-4 premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 3

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SDS-PAGE of Human FGF-4, premium grade under reduced (R) and non-reduced (NR) conditions.

Figure 3

SDS-PAGE of Human FGF-4, premium grade under reduced (R) and non-reduced (NR) conditions.

Figure 4

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Mass spectrometry analysis (ESI-MS) of Human FGF-4, premium grade. The peak corresponds to the calculated molecular mass of 19292 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human FGF-4, premium grade. The peak corresponds to the calculated molecular mass of 19292 Da.

Specifications for Human FGF-4

Overview

FGF-4 stands for fibroblast growth factor 4. Human FGF-4 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human FGF-4 can be used for a variety of applications, including:
  • Promotion of embryonic stem cell proliferation and differentiation.
  • Culture of fibroblast and epithelial cells and investigation of angiogenesis.
  • Study of FGFR and SHH signaling pathways.

Alternative names

HBGF-4

Detailed product information

Background information

Fibroblast growth factor 4 (FGF-4) is a heparin-binding growth factor and member of the FGF family, which controls several physiological and pathological development processes, including cell growth, cell survival, and morphogenesis. FGF-4 is expressed during embryo development and in several tissues during the adult life, and binds both receptors FGFR 1c, 2c, 3c, and 4. FGF-4 function partially overlaps with the one of FGF-8. By promoting cell differentiation, FGF-4 plays a central role during prenatal development and tissue regeneration, but can also have a pathological role as oncogene in tumor growth. Increased levels of FGF-4 was found in several human tumors. Altered levels of FGF-4 during embryo development can result in abnormal limb formation. Mature human FGF-4 shares 86% amino acid identity with mouse FGF-4.

Biological activity

  • Proliferation of mouse 3T3 cells
  • research grade: ≥ 5×
    10
    5
    U/mg
  • premium grade: ≥ 1×
    10
    6
    U/mg
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human FGF-4

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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