Clone:
REA810
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Ly-6G, Ly-6G.1

Extended validation for Gr-1 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA810
REA526+
1A8+
RB6-8C5++
Cells were incubated with an excess of purified unconjugated Gr-1 (REA810) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Anti-Gr-1. Bone marrow cells from C57BL/6 mice were stained with Anti-Gr-1 antibodies and with a suitable counterstaining. As a control, Anti-Gr-1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-Gr-1. Bone marrow cells from C57BL/6 mice were stained with Anti-Gr-1 antibodies and with a suitable counterstaining. As a control, Anti-Gr-1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-Gr-1. Bone marrow cells from C57BL/6 mice were stained with Anti-Gr-1 antibodies and with a suitable counterstaining. As a control, Anti-Gr-1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Anti-Gr-1. Bone marrow cells from C57BL/6 mice were stained with Anti-Gr-1 antibodies and with a suitable counterstaining. As a control, Anti-Gr-1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using Gr-1 (REA810). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using Gr-1 (REA810). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using Gr-1 (REA810). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for Gr-1 Antibody, anti-mouse, REAfinity™

Overview

Clone REA810 recognizes the mouse granulocyte-differentiation antigen-1 (Gr-1) antigen, also known as Ly-6G, a 21–25 kDa, GPI-anchored cell surface protein. Cross-reactivity of Gr-1 with Ly-6C was not detected on hematopoietic cells that express Ly-6C and are negative for Ly-6G. Gr-1 is expressed on mature granulocytes in bone marrow and peripheral tissues. The Anti-Gr-1 antibody also stains monocytes transiently during their differentiation in bone marrow and at low levels plasmacytoid dendritic cells in lymphoid tissues.
Additional information: Clone REA810 displays negligible binding to Fc receptors.

Alternative names

Ly-6G, Ly-6G.1

Detailed product information

Technical specifications

CloneREA810
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenGr-1
Alternative names of antigenLy-6G, Ly-6G.1
Molecular mass of antigen [kDa]10
Distribution of antigengranulocytes, monocytes, bone marrow
Entrez Gene ID546644
RRIDAB_2651869, AB_2651870, AB_2651871, AB_2651872, AB_2651873, AB_2651874, AB_2651875, AB_2651876, AB_2651877, AB_2651878, AB_2651879, AB_2651880, AB_2651881, AB_2651882, AB_2651883, AB_2651884, AB_2651868

Resources for Gr-1 Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for Gr-1 Antibody, anti-mouse, REAfinity™

Publications

  1. Fleming, T. J. et al. (1993) Characterization of two novel Ly-6 genes. Protein sequence and potential structural similarity to alpha-bungarotoxin and other neurotoxins. J. Immunol. 150(12): 5379-5390
  2. Fleming, T. J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J. Immunol. 151: 2399-2408
  3. Nagendra, S. and Schlueter, A. J. (2004) Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A 58: 195-200

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