Clone:
CRA1
Type of antibody:
Primary antibodies, Functional-grade antibodies
Isotype:
mouse IgG2bκ, mouse IgG2b
Applications:
FC, MICS, IF, IHC, FA
Alternative names:
FCER1A, Fce1a, FcERI

Extended validation for FcεRIα Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with CRA1
REA758++
Cells were incubated with an excess of purified unconjugated FcεRIα (CRA1) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using FcεRIα (CRA1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using FcεRIα (CRA1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using FcεRIα (CRA1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for FcεRIα Antibody, anti-human

Overview

Clone CRA1 detects the α subunit of the human high-affinity Fc receptor for IgE. The receptor is composed of one α-, one β-, and two disulphide-linked γ-chains, of which the α subunit binds IgE1. FcεRIα is expressed on mast and basophil cells, is upregulated in the presence of IgE, and is found on a subset of blood dendritic cells. CRA1 does not inhibit the binding of IgE it induces mast cell activation followed by a histamine release. The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.

Alternative names

FCER1A, Fce1a, FcERI

Detailed product information

Technical specifications

CloneCRA1
Clonalitymonoclonal
Isotypemouse IgG2bκ, mouse IgG2b
Isotype controlIsotype Control Antibody, mouse IgG2b
Hostmouse
Type of antibodyPrimary antibodies, Functional-grade antibodies
Specieshuman
AntigenFcεRIα
Alternative names of antigenFCER1A, Fce1a, FcERI
Molecular mass of antigen [kDa]27
Entrez Gene ID2205
RRIDAB_2751381, AB_2819473, AB_2819458, AB_10828822, AB_2660603, AB_10839418, AB_10828234, AB_2660604, AB_2660605, AB_2660606, AB_2660607, AB_2660608, AB_2660609, AB_10828815, AB_2751392

Resources for FcεRIα Antibody, anti-human

Certificates

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References for FcεRIα Antibody, anti-human

Publications

  1. Hasegawa, S. et al. (1999) Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its`intracellular expression in human megakaryocytes. Blood 93: 2543-2551
  2. Hammad, H. et al. (2010) Inflammatory dendritic cells–not basophils–are necessary and sufficient for induction of Tʜ2 immunity to inhaled house dust mite allergen. J. Exp. Med. 207: 2097-2111
  3. Suzukawa, M. et al. (2005) IgE- and FcepsilonRI-mediated migration of human basophils. Int. Immunol. 17: 1249-1255
  4. Takai, T. et al. (2000) Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions. Biosci. Biotechnol. Biochem. 64: 1856-1867
  5. Yokoi, H. et al. (2008) Inhibition of Fcepsilon RI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement. J. Allergy Clin. Immunol. 121: 499-505
  6. Hakimi, J. et al. (1990) The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding. J. Biol. Chem. 265: 22079-22081

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