Endotoxin Removal Beads represent the ideal tool for fast and easy removal of endotoxin and DNA from purified protein preparations.
Preparations of recombinant proteins are often contaminated with endotoxins (lipopolysaccharides, LPS) and DNA from host bacteria. Bacterial endotoxins and DNA show strong biological effects in many mammals as well as in cell cultures. If purified recombinant proteins are used for
in vivo
or
in vitro
assays, efficient removal of endotoxins and bacterial DNA is required to avoid non-specific detrimental effects.
The covalently bound polycationic moiety of the

Data and images for Endotoxin Removal Beads

Figures

Figure 1

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Removal of various endotoxins from a 1.0 mg/mL BSA solution in 0.1 mol/L sodium-phosphate buffer. The Endotoxin Removal Bead–concentration was 15 mg/mL. BSA recovery was above 84% at any rate.

Figure 1

Removal of various endotoxins from a 1.0 mg/mL BSA solution in 0.1 mol/L sodium-phosphate buffer. The Endotoxin Removal Bead–concentration was 15 mg/mL. BSA recovery was above 84% at any rate.

Figure 2

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Correlation of endotoxin removal and protein recovery. The Endotoxin Removal Bead concentration was 15 mg per mL of 0.1 M Na
2
HPO
4
buffer. The incubation time was 10 minutes.

Figure 2

Correlation of endotoxin removal and protein recovery. The Endotoxin Removal Bead concentration was 15 mg per mL of 0.1 M Na
2
HPO
4
buffer. The incubation time was 10 minutes.

Specifications for Endotoxin Removal Beads

Overview

Endotoxin Removal Beads represent the ideal tool for fast and easy removal of endotoxin and DNA from purified protein preparations.
Preparations of recombinant proteins are often contaminated with endotoxins (lipopolysaccharides, LPS) and DNA from host bacteria. Bacterial endotoxins and DNA show strong biological effects in many mammals as well as in cell cultures. If purified recombinant proteins are used for
in vivo
or
in vitro
assays, efficient removal of endotoxins and bacterial DNA is required to avoid non-specific detrimental effects.
The covalently bound polycationic moiety of the Endotoxin Removal Beads shows strong binding to both endotoxins and DNA.

Detailed product information

Background information

Endotoxin or DNA can be removed by 98% at initial concentrations ranging from 2000 EU/mL to 10,000 EU/mL or 2000 to 10,000 ng of DNA per mL, respectively. Endotoxin removal is performed using the MACSiMAG™ Separator.
Note: Endotoxin Removal Beads are
not
suitable for use with MACS® Columns and MiniMACS™, MidiMACS™, VarioMACS™, SuperMACS™, autoMACS®, μMACS™, or thermoMACS™ Separators.

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