CD22 antibodies

CD22 antibodies

Clone: REA340
Clone REA340 recognizes the human CD22 antigen, a single-pass type I membrane protein which is also known as sialic acid-binding Ig-like lectin 2 (Siglec-2). In peripheral blood, cell surface expression of CD22 is found on mature B cells, but is lost during terminal differentiation to plasma cells. In bone marrow, surface expression of CD22 can be detected from the pre–B cell stage on. Cytoplasmic expression of CD22 can also be found in late pro–B cells and early–B cells. CD22 plays a strong role in cell interactions and B cell activation. It is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases. CD22 has emerged as an ideal target for monoclonal antibody based therapy of B cell malignancies including most lymphomas and many leukemias.
Additional information: Clone REA340 displays negligible binding to Fc receptors.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.
Expression of CD22 may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Alternative names

SIGLEC-2, B-lymphocyte cell adhesion molecule (BL-CAM), Sialic acid-binding Ig-like lectin 2 (Siglec-2)

Technical specifications

  • Antigen: CD22
  • Clone: REA340
  • Isotype: recombinant human IgG1
  • Alternative names of antigen: SIGLEC-2, B-lymphocyte cell adhesion molecule (BL-CAM), Sialic acid-binding Ig-like lectin 2 (Siglec-2)
  • Molecular mass of antigen [kDa]: 93
  • Distribution of antigen: B cells, bone marrow
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBlue, APC

  • Figure 1

    Human EDTA-treated whole blood samples were stained with CD22 antibodies as well as CD19 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant
    ®
    Analyzer. For analysis, lymphocytes were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence.

  • Selected references

    1. Stamenkovic, I. et al. (1990) The B-cell antigen CD22 mediates monocyte and erythrocyte adhesion. Nature 345(6270): 74-77
    2. Wilson, G. L. et al. (1991) cDNA cloning of the B cell membrane protein CD22: a mediator of B-B cell interactions. J. Exp. Med. 173(1): 137-146
    3. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
    4. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
    5. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
    6. Sullivan-Chang, L. et al. (2013) Targeting CD22 in B-cell malignancies: current status and clinical outlook. BioDrugs 27(4): 293-304
    7. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
    8. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition

  • Brochures and posters

  • Certificates

    Please follow this
    link
    to download the Certificate of Conformity (CoC) by lot number.
Product options: 2
170-078-019

CD22-APC

for 100
tests
EUR 460,00 
170-078-018

CD22-VioBlue

for 100
tests
EUR 475,00 

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