Clone:
3B2/TA8
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2aκ
Applications:
FC, MICS, IF, IHC
Alternative names:
HBA71, MIC2, MIC2X, MIC2Y, MSK5X

Extended validation for CD99 Antibody, anti-human

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD99 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD99-PE (3B2/TA8, red) and counterstained with DRAQ5 (blue) as DNA stain.
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Fluorescence microscopy image of CD99 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD99-PE (3B2/TA8, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD99 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD99-PE (3B2/TA8, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD99 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD99-PE, clone (3B2/TA8). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD99 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD99-PE, clone (3B2/TA8). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD99 Antibody, anti-human

Overview

Clone 3B2/TA8 recognizes the human CD99 antigen, a 32 kDa type I single chain transmembrane sialoglycoprotein which is also known as E2 antigen or MIC2. The expression level of CD99 is particularly high in thymocytes, T cells, T cell leukemias and lymphomas, subsets of mature plasma cells in the medullary cord of reactive lymph nodes, pancreatic islet cells, granulosa cells of the ovary, Sertoli cells of the testes, but absent on granulocytes. It is involved in T cell–adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane and it acts at the same site as, but independently of, PECAM1.

Alternative names

HBA71, MIC2, MIC2X, MIC2Y, MSK5X

Detailed product information

Technical specifications

Clone3B2/TA8
Clonalitymonoclonal
Isotypemouse IgG2aκ
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD99
Alternative names of antigenHBA71, MIC2, MIC2X, MIC2Y, MSK5X
Molecular mass of antigen [kDa]17
Distribution of antigenT cells, plasma cells, thymocytes
Entrez Gene ID4267
RRIDAB_2876911, AB_2857658, AB_2857633, AB_2857695, AB_2857683, AB_2659698, AB_2659699, AB_2659700, AB_2659701, AB_2659702, AB_2659703, AB_2876910

Resources for CD99 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD99 Antibody, anti-human

Publications

  1. Schenkel, A. R. et al. (2002) CD99 plays a major role in the migration of monocytes through endothelial junctions. Nat. Immunol. 3(2): 143-150
  2. Gelin, C. et al. (1989) The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product. EMBO J. 8(11): 3253-3259
  3. Edlund, K. et al. (2012) CD99 is a novel prognostic stromal marker in non-small cell lung cancer. Int. J. Cancer 131(10): 2264-2273

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