Clone:
REA897
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Thy-1, THY1, CDw90

Extended validation for CD90 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA897
5E10++
Thy-1A1n/a
F15-42-1++
DG3+
REAL449+
REAL677n/a
Cells were incubated with an excess of purified unconjugated CD90 (REA897) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD90 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD90-PE (REA897, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD90 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD90-PE (REA897, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD90 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD90-PE (REA897, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD90 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD90-PE, clone (REA897). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD90 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD90-PE, clone (REA897). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD90 (REA897). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD90 (REA897). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD90 (REA897). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD90 (REA897). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD90 (REA897). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD90 Antibody, anti-human, REAfinity™

Overview

Clone REA897 recognizes the human CD90 antigen, which is a 25–35 kD GPI-anchored protein of the Ig superfamily. It is expressed on neurons, small subsets of human fetal liver cells and thymocytes, cord blood, and bone marrow cells. CD90 is also expressed on a subset of CD34
+
primitive hematopoietic stem cells. CD90
+
CD34
+
cells characterize a highly enriched population of high proliferative potential colony-forming cells (HPP-CFC). Furthermore, CD90 expression is found on mesenchymal stromal cells (MSCs). CD90, also known as Thy-1, is involved in regulation of adhesion and signal transduction of T cells.
Additional information: Clone REA897 displays negligible binding to Fc receptors.

Alternative names

Thy-1, THY1, CDw90

Detailed product information

Technical specifications

CloneREA897
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
cynomolgus monkey (
Macaca fascicularis
)
,
rhesus monkey (
Macaca mulatta
)
, baboon, pigtailed macaque
AntigenCD90
Alternative names of antigenThy-1, THY1, CDw90
Molecular mass of antigen [kDa]13
Distribution of antigenstem cells, endothelial cells, fibroblasts, leukocytes, lymphocytes, neurons, ILC, T cells, cancer stem cells, hematopoietic stem and progenitor cells, leukemia cells, mesenchymal stem cells, ES and iPS cells, thymocytes, brain
Entrez Gene ID7070
RRIDAB_2726810, AB_2726842, AB_2726811, AB_2726843, AB_2726812, AB_2726847, AB_2726816, AB_2726848, AB_2726817, AB_2726844, AB_2726813, AB_2726845, AB_2726814, AB_2726846, AB_2726815, AB_2726809, AB_2726841

Resources for CD90 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD90 Antibody, anti-human, REAfinity™

Publications

  1. Mayani, H. and Lansdorp, P. M. (1994) Thy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood. Blood 83: 2410-2417
  2. Wetzel, A. et al. (2004) Human Thy-1 (CD90) on activated endothelial cells is a counterreceptor for the leukocyte integrin Mac-1 (CD11b/CD18). J. Immunol. 172(6): 3850-3859
  3. Dominici, M. et al. (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8: 315-317

Related products for
CD90 Antibody, anti-human, REAfinity™

2 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?