Clone:
REA983
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
B7-1, Cd28l, Ly-53, Ly53, MIC17, TSA1, B7, BB1

Extended validation for CD80 Antibody, anti-mouse, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD80 (REA983). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD80 (REA983). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD80 (REA983). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD80 (REA983). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD80 (REA983). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD80 Antibody, anti-mouse, REAfinity™

Overview

Clone REA983 recognizes the mouse CD80 antigen also known as B7, B7-1, or Ly-53. The 55 kDa member of the Ig superfamily is expressed on activated B cells, macrophages, and dendritic cells. The interaction of CD80 and CD152 (CTLA-4) or CD28 induces T cell proliferation and cytokine production.
Additional information: Clone REA983 displays negligible binding to Fc receptors.

Alternative names

B7-1, Cd28l, Ly-53, Ly53, MIC17, TSA1, B7, BB1

Detailed product information

Technical specifications

CloneREA983
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse, other
Cross-reactivitydog
AntigenCD80
Alternative names of antigenB7-1, Cd28l, Ly-53, Ly53, MIC17, TSA1, B7, BB1
Molecular mass of antigen [kDa]30
Distribution of antigenB cells, dendritic cells, Langerhans cells, macrophages, monocytes, T cells, Langerhans cells
Entrez Gene ID12519
RRIDAB_2727513, AB_2727557, AB_2727514, AB_2727558, AB_2727515, AB_2727563, AB_2727520, AB_2727559, AB_2727516, AB_2727560, AB_2727517, AB_2727561, AB_2727518, AB_2727562, AB_2727519, AB_2727556

Resources for CD80 Antibody, anti-mouse, REAfinity™

Certificates

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References for CD80 Antibody, anti-mouse, REAfinity™

Publications

  1. Razi-Wolf, Z. et al. (1992) Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells. Proc. Natl. Acad. Sci. U.S.A. 89: 4210 -4214
  2. Harlan, D. M. et al. (1994) Mice expressing both B7-1 and viral glycoprotein on pancreatic beta cells along with glycoprotein-specific transgenic T cells develop diabetes due to a breakdown of T-lymphocyte unresponsiveness. Proc. Natl. Acad. Sci. U.S.A. 91: 3137-3141
  3. Hathcock, K. S et al. (1994) Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function. J. Exp. Med. 180(2): 631-640
  4. Bluestone, J. A. et al. (1995) New perspectives of CD28-B7-mediated T cell costimulation. Immunity 2(6): 555-559

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