Clone:
16-10A1
Type of antibody:
Primary antibodies
Isotype:
hamster IgG2κ
Applications:
FC
Alternative names:
B7-1, Cd28l, Ly-53, Ly53, MIC17, TSA1, B7, BB1

Extended validation for CD80 Antibody, anti-mouse

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD80 (16-10A1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD80 (16-10A1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD80 (16-10A1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD80 Antibody, anti-mouse

Overview

The monoclonal antibody 16-10A1 recognizes mouse CD80 also known as B7, B7-1 or Ly-53. The 55 kDa member of the Ig superfamily is expressed on activated B cells, macrophages and dendritic cells. The interaction of CD80 and CD152 (CTLA-4) or CD28 induces T cell proliferation and cytokine production.

Alternative names

B7-1, Cd28l, Ly-53, Ly53, MIC17, TSA1, B7, BB1

Detailed product information

Technical specifications

Clone16-10A1
Clonalitymonoclonal
Isotypehamster IgG2κ
Hosthamster
Type of antibodyPrimary antibodies
Speciesmouse, other
Cross-reactivitydog
AntigenCD80
Alternative names of antigenB7-1, Cd28l, Ly-53, Ly53, MIC17, TSA1, B7, BB1
Molecular mass of antigen [kDa]30
Distribution of antigenB cells, dendritic cells, macrophages, monocytes, Langerhans cells, T cells
Entrez Gene ID12519
RRIDAB_2659264, AB_2659265, AB_2659266, AB_2659267, AB_2659268, AB_2659269, AB_2659270, AB_2659271, AB_2659272, AB_2659273

Resources for CD80 Antibody, anti-mouse

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

Reviews for CD80 Antibody, anti-mouse

Staining CD80 in Skin

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CD80-PE, mouse (130-102-613)

It works well to detect targeted cells in the skin.

Staining CD80 in Skin

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CD80-PE, mouse (130-102-883)

It works well to detect targeted cells in the skin.

References for CD80 Antibody, anti-mouse

Publications

  1. Bluestone, J. A. et al. (1995) New perspectives of CD28-B7-mediated T cell costimulation. Immunity 2(6): 555-559
  2. Hathcock, K. S et al. (1994) Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function. J. Exp. Med. 180(2): 631-640
  3. Harlan, D. M. et al. (1994) Mice expressing both B7-1 and viral glycoprotein on pancreatic beta cells along with glycoprotein-specific transgenic T cells develop diabetes due to a breakdown of T-lymphocyte unresponsiveness. Proc. Natl. Acad. Sci. U.S.A. 91: 3137-3141
  4. Razi-Wolf, Z. et al. (1992) Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells. Proc. Natl. Acad. Sci. U.S.A. 89: 4210 -4214

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