Clone:
REA886
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MICS, IF, IHC, MC
Alternative names:
gp110, Lamp4, SCARD1, macrosialin

Extended validation for CD68 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA886
Y1/82A++
Cells were incubated with an excess of purified unconjugated CD68 (REA886) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD68. Human peripheral blood mononuclear cells (PBMCs) were stained with a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD68. Human peripheral blood mononuclear cells (PBMCs) were stained with a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD68. Human peripheral blood mononuclear cells (PBMCs) were stained with a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD68. Human peripheral blood mononuclear cells (PBMCs) were stained with a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD68. Human peripheral blood mononuclear cells (PBMCs) were stained with a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD68 Antibody, anti-human, REAfinity™

Overview

Clone REA886 recognizes the human CD68 antigen, a 110 kDa type I transmembrane glycoprotein classified as a lysosomal/endosomal–associated membrane glycoprotein (LAMP) family member. CD68 is highly expressed by human monocytes and tissue macrophages but also found in subpopulations of B cells, fibroblasts, endothelial cells, and other cells. It is found predominantly in late endosomal and lysosomal compartments, with a modest level of cell surface expression. CD68 is suggested to be involved in phagocytic activities of tissue macrophages, in intracellular lysosomal metabolism, and extracellular cell-cell and cell-pathogen interactions.
Additional information: Clone REA886 displays negligible binding to Fc receptors.

Alternative names

gp110, Lamp4, SCARD1, macrosialin

Detailed product information

Technical specifications

CloneREA886
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
cynomolgus monkey (
Macaca fascicularis
)
AntigenCD68
Alternative names of antigengp110, Lamp4, SCARD1, macrosialin
Molecular mass of antigen [kDa]35
Distribution of antigenmonocytes, macrophages, B cells, fibroblasts, endothelial cells
Entrez Gene ID968
RRIDAB_2726727, AB_2726642, AB_2726728, AB_2726643, AB_2726731, AB_2726646, AB_2726729, AB_2726644, AB_2726730, AB_2726645

Resources for CD68 Antibody, anti-human, REAfinity™

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for CD68 Antibody, anti-human, REAfinity™

Publications

  1. Saito, N. et al. (1991) Ultrastructural localization of the CD68 macrophage-associated antigen in human blood neutrophils and monocytes. Am. J. Pathol. 139: 1053-1059
  2. Holness, C. L. and Simmons, D. L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood 81: 1607-1613
  3. Holness, C. L. et al. (1993) Macrosialin, a mouse macrophage-restricted glycoprotein, is a member of the lamp/lgp family. J. Biol. Chem. 268: 9661-9666
  4. Kurushima, H. et al. (2000) Surface expression and rapid internalization of macrosialin (mouse CD68) on elicited mouse peritoneal macrophages. J. Leukoc. Biol. 67: 104-108
  5. Gottfried, L. A. et al. (2008) Expression of CD68 in non-myeloid cell types. Scand. J. Immunol. 67: 453-463

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