Clone:
REA1099
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
ITGAV, MSK8, VNRA, VTNR, BDPLT16, BDPLT2, CD61, GP3A, GPIIIa, ITGB3

Extended validation for CD51/CD61 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1099
23C6++
NKI-M9-
P1F6-
REA181++
Cells were incubated with an excess of purified unconjugated CD51/CD61 (REA1099) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD51/CD61. Human fibroblast cells were stained with CD51/CD61 antibodies and plotted against the side scatter. As a control, CD51/CD61 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD51/CD61. Human fibroblast cells were stained with CD51/CD61 antibodies and plotted against the side scatter. As a control, CD51/CD61 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD51/CD61. Human fibroblast cells were stained with CD51/CD61 antibodies and plotted against the side scatter. As a control, CD51/CD61 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD51/CD61. Human fibroblast cells were stained with CD51/CD61 antibodies and plotted against the side scatter. As a control, CD51/CD61 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD51/CD61. Human fibroblast cells were stained with CD51/CD61 antibodies and plotted against the side scatter. As a control, CD51/CD61 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD51/CD61 (REA1099). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD51/CD61 (REA1099). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD51/CD61 (REA1099). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD51/CD61 Antibody, anti-human, REAfinity™

Overview

Clone REA1099 recognizes the human heterodimeric complex CD51/CD61, also known as integrin αv/β3. The dimers are expressed on melanoma cells, endothelial cells, on osteoclasts, and in a very low density on platelets. The CD51/CD61 complex mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, osteopontin, and collagen.
Additional information: Clone REA1099 displays negligible binding to Fc receptors.

Alternative names

ITGAV, MSK8, VNRA, VTNR, BDPLT16, BDPLT2, CD61, GP3A, GPIIIa, ITGB3

Detailed product information

Technical specifications

CloneREA1099
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, other
Cross-reactivityrabbit, chicken
AntigenCD51/CD61
Alternative names of antigenITGAV, MSK8, VNRA, VTNR, BDPLT16, BDPLT2, CD61, GP3A, GPIIIa, ITGB3
Molecular mass of antigen [kDa]197
Distribution of antigenendothelial cells, osteoclasts, platelets, melanoma, melanoma
Entrez Gene ID3685, 3690
RRIDAB_2751665, AB_2751617, AB_2751666, AB_2751618, AB_2751667, AB_2751619, AB_2751668, AB_2751620

Resources for CD51/CD61 Antibody, anti-human, REAfinity™

Certificates

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to search for Certificates of Analysis (CoA) by lot number.

References for CD51/CD61 Antibody, anti-human, REAfinity™

Publications

  1. Athanasou, N. A. et al. (1990) New sites of cellular vitronectin receptor immunoreactivity detected with osteoclast-reacting monoclonal antibodies 13C2 and 23C6. Bone Miner. 8(1): 7-22
  2. Chuntharapai, A. et al. (1993) Blocking monoclonal antibodies to alpha V beta 3 integrin: a unique epitope of alpha V beta 3 integrin is present on human osteoclasts. Exp. Cell Res. 205(2): 345-352

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