Clone:
REAL396
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC, MICS, IHC, IF
Alternative names:
Intercellular adhesion molecule 3, CDw50, ICAM-R, ICAM3

Extended validation for CD50 (ICAM-3) Antibody, anti-human,
REAlease
®

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD50 (ICAM-3) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD50 (ICAM-3)-PE (REAL396, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD50 (ICAM-3) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD50 (ICAM-3)-PE (REAL396, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD50 (ICAM-3) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD50 (ICAM-3)-PE (REAL396, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD50 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD50-PE, clone (REAL396). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD50 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD50-PE, clone (REAL396). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD50 (ICAM-3) Antibody, anti-human,
REAlease
®

Overview

Clone REAL396 is an antibody fragment derived from the full CD50 (ICAM-3) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL396 recognizes the human CD50 antigen, a type I transmembrane glycoprotein also known as ICAM-3 (Intercellular adhesion molecule 3). CD50, a member of the immunoglobulin superfamily, is closely related to ICAM-1 and consists of five immunoglobulin domains. CD50 is expressed on leukocytes. CD50 is a ligand for CD11a (leukocyte adhesion LFA-1 protein) and is also associated with the amino-terminal domain of ERM proteins and moesin. It plays an essential role in primary immune responses.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

Intercellular adhesion molecule 3, CDw50, ICAM-R, ICAM3

Detailed product information

Technical specifications

CloneREAL396
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD50 (ICAM-3)
Alternative names of antigenIntercellular adhesion molecule 3, CDw50, ICAM-R, ICAM3
Distribution of antigenleukocytes
RRIDAB_2819385, AB_2819402, AB_2819399, AB_2819386

Resources for CD50 (ICAM-3) Antibody, anti-human,
REAlease
®

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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