Clone:
REAL377
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC, MICS, IF, IHC

Extended validation for CD45RO Antibody, anti-human,
REAlease
®

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD45RO knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD45RO-PE (REAL377, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD45RO knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD45RO-PE (REAL377, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD45RO knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD45RO-PE (REAL377, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of human CD45RO knockout cells. Wild type (red) and knockout cells (blue) were stained with CD45RO-PE, clone (REAL377). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of human CD45RO knockout cells. Wild type (red) and knockout cells (blue) were stained with CD45RO-PE, clone (REAL377). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD45RO Antibody, anti-human,
REAlease
®

Overview

Clone REAL377 is an antibody fragment derived from the full CD45RO antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL377 recognizes the human CD45RO antigen, a 180 kDa single-chain membrane glycoprotein, which is an isoform of CD45. CD45RO is expressed by most thymocytes, activated and memory T cells, granulocytes, B cell subsets, and monocytes. CD45RO binds to galectin-1 and CD22. CD45RO antibodies are commonly used in combination with antibodies against CD45RA to discriminate memory T cells from naive T cells.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Detailed product information

Technical specifications

CloneREAL377
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD45RO
Distribution of antigengranulocytes, T cells, B cells, monocytes, thymocytes
RRIDAB_2784223, AB_2784222, AB_2784221, AB_2811684, AB_2811683, AB_2784224

Resources for CD45RO Antibody, anti-human,
REAlease
®

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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