Clone:
REA1180
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Ptprc, B220, CD45R, CD45, L-CA, Ly-5, Lyt-4, T200, loc

Extended validation for CD45RB Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1180
16A++
C363-16A++
MB4B4++
Cells were incubated with an excess of purified unconjugated CD45RB (REA1180) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD45RB. C57BL/6 splenocytes were stained with CD45RB antibodies and plotted against the side scatter. As a control, CD45RB antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD45RB. C57BL/6 splenocytes were stained with CD45RB antibodies and plotted against the side scatter. As a control, CD45RB antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD45RB. C57BL/6 splenocytes were stained with CD45RB antibodies and plotted against the side scatter. As a control, CD45RB antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD45RB. C57BL/6 splenocytes were stained with CD45RB antibodies and plotted against the side scatter. As a control, CD45RB antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD45RB. C57BL/6 splenocytes were stained with CD45RB antibodies and plotted against the side scatter. As a control, CD45RB antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD45RB. C57BL/6 splenocytes were stained with CD45RB antibodies and plotted against the side scatter. As a control, CD45RB antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD45RB (REA1180). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD45RB (REA1180). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD45RB (REA1180). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD45RB Antibody, anti-mouse, REAfinity™

Overview

Clone REA1180 recognizes an isoform of mouse CD45 known as CD45RB, which is exon B dependent. It is a 220 kDa glycoprotein found in a high density on peripheral B cells, on a subpopulation of T cells and thymocytes and is weakly expressed on macrophages and dendritic cells. CD45 is a member of the protein tyrosine phosphatase (PTP) family.
Additional information: Clone REA1180 displays negligible binding to Fc receptors.

Alternative names

Ptprc, B220, CD45R, CD45, L-CA, Ly-5, Lyt-4, T200, loc

Detailed product information

Technical specifications

CloneREA1180
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD45RB
Alternative names of antigenPtprc, B220, CD45R, CD45, L-CA, Ly-5, Lyt-4, T200, loc
Molecular mass of antigen [kDa]134
Distribution of antigenB cells, macrophages, monocytes, granulocytes, mast cells, dendritic cells, T cells, NK cells, basophils, thymocytes, plasma cells
Entrez Gene ID19264
RRIDAB_2811359, AB_2811366, AB_2811360, AB_2811367, AB_2811361, AB_2811368, AB_2811362, AB_2811369, AB_2811363, AB_2811370, AB_2811364, AB_2811365

Resources for CD45RB Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for CD45RB Antibody, anti-mouse, REAfinity™

Publications

  1. Birkeland, M. L. et al. (1988) Epitopes on CD45R [T200] molecules define differentiation antigens on murine B and T lymphocytes. J. Mol. Cell. Immunol 4(2): 71-85
  2. Pulido, R. et al. (1988) Comparative biochemical and tissue distribution study of four distinct CD45 antigen specificities. J. Immunol. 140(11): 3851-3857
  3. Johnson, P. et al. (1989) Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies. J. Exp. Med. 169: 1179

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