Mouse CD45R (B220) MicroBeads were developed for the positive selection or depletion of mouse B cells from various lymphoid tissues like bone marrow, lymph nodes, and spleen or from non-lymphoid sources like lamina propria, peritoneal cavity, or lung.

Data and images for CD45R (B220) MicroBeads, mouse

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Figure 1

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Isolation of B cells from mouse spleen cell suspension using CD45R MicroBeads, a MiniMACS™ Separator, and an MS Column.

Figure 1

Isolation of B cells from mouse spleen cell suspension using CD45R MicroBeads, a MiniMACS™ Separator, and an MS Column.

Specifications for CD45R (B220) MicroBeads, mouse

Overview

Mouse CD45R (B220) MicroBeads were developed for the positive selection or depletion of mouse B cells from various lymphoid tissues like bone marrow, lymph nodes, and spleen or from non-lymphoid sources like lamina propria, peritoneal cavity, or lung.

Detailed product information

Background information

The CD45R antigen is expressed on B lineage cells throughout their development from early pro-B cell stages on and is down-regulated upon terminal differentiation to plasma cells. CD45R is also expressed on plasmacytoid dendritic cells.

Downstream applications

B cells purified with CD45R MicroBeads have been used for studies on the mechanism and regulation of immunoglobulin gene rearrangement,
1
for analyses of signal transduction,
2,3
and for reconstitution of immunodeficient and parasite-infected mice.
4

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

Resources for CD45R (B220) MicroBeads, mouse

Documents and Protocols

References for CD45R (B220) MicroBeads, mouse

Publications

  1. Maki, K. et al. (2000) Immunoglobulin beta signaling regulates locus accessibility for ordered immunoglobulin gene rearrangements. J. Exp. Med. 191: 1333-1340
  2. Weintraub, B. C. et al. (2000) Entry of B cell receptor into signaling domains is inhibited in tolerant B cells. J. Exp. Med. 191: 1443-1448
  3. Suzuki, H. et al. (1999) Xid-like immunodeficiency in mice with disruption of the p85alpha subunit of phosphoinositide 3-kinase. Science 283: 390-392
  4. Hoerauf, A. et al. (1996)
    Co-transfer of B cells converts resistance into susceptibility in T cell-reconstituted,
    Leishmania major
    -resistant C.B-17 scid mice by a non-cognate mechanism.
    Int. Immunol. 8: 1569-1575

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