Clone:
REAL469
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC
Alternative names:
Ptprc, B220, CD45, CD45R, L-CA, Ly-5, Lyt-4, T200, loc, Ly-5.1

Extended validation for CD45.1 Antibody, anti-mouse,
REAlease
®

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REAL469
A20++
REA1179++
Cells were incubated with an excess of purified unconjugated CD45.1 (REAL469) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD45.1 Antibody, anti-mouse,
REAlease
®

Overview

Clone REAL469 is an antibody fragment derived from the full CD45.1 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL469 recognizes the mouse CD45.1 alloantigen of CD45, a membrane glycoprotein also known as leukocyte common antigen or Ly-5. CD45.1 is expressed by all leukocytes in mouse strains SJL/J, STS/A, RIII, and DA. CD45 is a member of the protein tyrosine phosphatase family and is involved in the regulation of T and B cell antigen receptor signaling. Clone REAL469 does not react with the CD45.2 alloantigen expressed by most mouse strains.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

Ptprc, B220, CD45, CD45R, L-CA, Ly-5, Lyt-4, T200, loc, Ly-5.1

Detailed product information

Technical specifications

CloneREAL469
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD45.1
Alternative names of antigenPtprc, B220, CD45, CD45R, L-CA, Ly-5, Lyt-4, T200, loc, Ly-5.1
Distribution of antigenleukocytes
RRIDAB_2819416, AB_2819415

Resources for CD45.1 Antibody, anti-mouse,
REAlease
®

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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