Clone:
DB105
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC, ICC
Alternative names:
CSPG9, EMCR III, HCELL, HUTCH-I, CD44s, H-CAM, Pgp-1

Extended validation for CD44 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with DB105
G44-26-
BJ18++
C44Mab-5-
IM7 (h/m)-
515+
MEM-85++
REA690++
IM7.8.1 (h/m)-
REAL259++
Cells were incubated with an excess of purified unconjugated CD44 (DB105) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

View details
Flow cytometric comparison of different clones for CD44. Human peripheral blood mononuclear cells (PBMCs) were stained with CD44 antibodies and with a suitable counterstaining. As a control, CD44 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD44. Human peripheral blood mononuclear cells (PBMCs) were stained with CD44 antibodies and with a suitable counterstaining. As a control, CD44 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD44. Human peripheral blood mononuclear cells (PBMCs) were stained with CD44 antibodies and with a suitable counterstaining. As a control, CD44 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD44. Human peripheral blood mononuclear cells (PBMCs) were stained with CD44 antibodies and with a suitable counterstaining. As a control, CD44 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD44. Human peripheral blood mononuclear cells (PBMCs) were stained with CD44 antibodies and with a suitable counterstaining. As a control, CD44 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD44. Human peripheral blood mononuclear cells (PBMCs) were stained with CD44 antibodies and with a suitable counterstaining. As a control, CD44 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD44. Human peripheral blood mononuclear cells (PBMCs) were stained with CD44 antibodies and with a suitable counterstaining. As a control, CD44 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD44. Human peripheral blood mononuclear cells (PBMCs) were stained with CD44 antibodies and with a suitable counterstaining. As a control, CD44 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD44. Human peripheral blood mononuclear cells (PBMCs) were stained with CD44 antibodies and with a suitable counterstaining. As a control, CD44 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD44. Human peripheral blood mononuclear cells (PBMCs) were stained with CD44 antibodies and with a suitable counterstaining. As a control, CD44 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD44 (DB105). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD44 (DB105). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD44 (DB105). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD44 Antibody, anti-human

Overview

Clone DB105 recognizes the CD44 antigen. CD44 is a marker for many types of cancer stem cells (CSCs), including breast CSCs that possess higher tumorigenicity and metastatic potential, colorectal, pancreatic, and prostate CSCs. In addition, expression was observed in several cancers as well as on carcinoma cell lines. Here, CD44 plays a role in cancer cell migration and matrix adhesion in response to a cellular microenvironment, thus enhancing cellular aggregation and tumor cell growth. CD44 is also expressed on mesodermal cells, such as hematopoietic, fibroblastic, and glial cells.

Alternative names

CSPG9, EMCR III, HCELL, HUTCH-I, CD44s, H-CAM, Pgp-1

Detailed product information

Technical specifications

CloneDB105
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
AntigenCD44
Alternative names of antigenCSPG9, EMCR III, HCELL, HUTCH-I, CD44s, H-CAM, Pgp-1
Molecular mass of antigen [kDa]79
Distribution of antigenstem cells, chondrocytes, endothelial cells, epithelial cells, leukocytes, lymphocytes, myeloid leukemia cells, red blood cells, T cells, cancer stem cells, CNS cells, mesenchymal stem cells, plasma cells, ES and iPS cells, bone marrow, kidney, skeletal muscle, skin
Entrez Gene ID960
RRIDAB_2726110, AB_2726388, AB_2726111, AB_2726385, AB_2726108, AB_2726390, AB_2726113, AB_2726389, AB_2726112, AB_2726386, AB_2726109, AB_2733522, AB_2733523, AB_2726387

Resources for CD44 Antibody, anti-human

Documents and Protocols

Brochures/posters

Cancer stem cells

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

Reviews for CD44 Antibody, anti-human

Excellent CD44 Antibody from Miltenyi Biotec

  • 1
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CD44-FITC, human (130-113-334)

Will buy and use it again. Recommendable!

References for CD44 Antibody, anti-human

Publications

  1. Al-Hajj, M. et al. (2003) Prospective identification of tumorigenic breast cancer cells. Proc. Natl. Acad. Sci. U.S.A. 100: 3983-3988
  2. Dalerba, P. et al. (2007) Phenotypic characterization of human colorectal cancer stem cells. Proc. Natl. Acad. Sci. U.S.A. 104: 10158-10163
  3. Li, C. P. et al. (2007) Identification of pancreatic cancer stem cells. Cancer Res. 67: 1030-1037
  4. Collins, A. T. et al. (2005) Prospective identification of tumorigenic prostate cancer stem cells. Cancer Res. 65: 10946-10951
  5. Aruffo, A. et al. (1990) CD44 is the principal cell surface receptor for hyaluronate. Cell 61: 1303-1313
  6. Patrawala, L. et al. (2006)
    Highly purified CD44
    +
    prostate cancer cells from xenograft human tumors are enriched in tumorigenic and metastatic progenitor cells.
    Oncogene 25: 1696-1708
  7. Wang, L. et al. (2013) Enrichment of prostate cancer stem-like cells from human prostate cancer cell lines by culture in serum-free medium and chemoradiotherapy. Int. J. Biol. Sci. 9(5): 472-479

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