Clone:
REA716
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
SRB1, CLA-1, CD36 antigen-like 1, SR-BI, SCARB1

Extended validation for CD36L1 (SR-BI) Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD36L1 (SR-BI) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD36L1 (SR-BI)-PE (REA716, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD36L1 (SR-BI) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD36L1 (SR-BI)-PE (REA716, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD36L1 (SR-BI) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD36L1 (SR-BI)-PE (REA716, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of human CD36L knockout cells. Wild type (red) and knockout cells (blue) were stained with CD36L-PE, clone (REA716). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of human CD36L knockout cells. Wild type (red) and knockout cells (blue) were stained with CD36L-PE, clone (REA716). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD36L1 (SR-BI) Antibody, anti-human, REAfinity™

Overview

Clone REA716 recognizes the human CD36L1 antigen, an integral membrane protein, also known as SR-BI. CD36L1 is widly expressed, e.g. on macrophages, dendritic cells, adrenocortical cells, adipocytes, and trophoblastic cells. In hepatocytes, CD36L1 acts as a receptor for hepatitis C. It has been reported, that CD36L1 is able to bind HCV envelope glycoprotein E2, participate in entry of HCV pseudotype particles, and modulate HCV infection. Furthermore, CD36L1 is a receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, vitamin E, phosphatidylserine, and apoptotic cells.
Additional information: Clone REA716 displays negligible binding to Fc receptors.

Alternative names

SRB1, CLA-1, CD36 antigen-like 1, SR-BI, SCARB1

Detailed product information

Technical specifications

CloneREA716
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD36L1 (SR-BI)
Alternative names of antigenSRB1, CLA-1, CD36 antigen-like 1, SR-BI, SCARB1
Molecular mass of antigen [kDa]61
Distribution of antigendendritic cells, macrophages
Entrez Gene ID949
RRIDAB_2657767, AB_2657768, AB_2657769, AB_2657766

Resources for CD36L1 (SR-BI) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD36L1 (SR-BI) Antibody, anti-human, REAfinity™

Publications

  1. Scarselli, E. et al. (2002) The human scavenger receptor class B type I is a novel candidate receptor for the hepatitis C virus. EMBO J. 21(19): 5017-5025
  2. Zeisel, M. B. et al. (2007) Scavenger receptor class B type I is a key host factor for hepatitis C virus infection required for an entry step closely linked to CD81. Hepatology 46(6): 1722-1731
  3. Valacchi, G. et al. (2011) Scavenger receptor class B type I: a multifunctional receptor. Ann. N. Y. Acad. Sci. 1229

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