Clone:
REA213
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
TREM1

Extended validation for CD354 (TREM-1) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA213
TREM-26++
6B1++
193015+
Cells were incubated with an excess of purified unconjugated CD354 (TREM-1) (REA213) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD354 (TREM-1). Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD354 (TREM-1) antibodies and with a suitable counterstaining. As a control, CD354 (TREM-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD354 (TREM-1). Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD354 (TREM-1) antibodies and with a suitable counterstaining. As a control, CD354 (TREM-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD354 (TREM-1). Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD354 (TREM-1) antibodies and with a suitable counterstaining. As a control, CD354 (TREM-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD354 (TREM-1). Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD354 (TREM-1) antibodies and with a suitable counterstaining. As a control, CD354 (TREM-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD354 (TREM-1). Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD354 (TREM-1) antibodies and with a suitable counterstaining. As a control, CD354 (TREM-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD354 (TREM-1) (REA213). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD354 (TREM-1) (REA213). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD354 (TREM-1) (REA213). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD354 (TREM-1) Antibody, anti-human, REAfinity™

Overview

Clone REA213 recognizes the human CD354 (TREM-1), a 30 kDa member of the triggering receptors expressed on myeloid cells (TREM) family of cell surface receptors. TREM receptors are immunoglobulin 'superfamily' members and contain a single variable-type immunoglobulin domain. Human TREM cluster is located on chromosome 6p21. Expression of TREM-1 is found on the cell surface of myeloid cells such as neutrophils, monocytes, dendritic cells, and macrophages. TREM-1 signals via ITAM-containing adaptor DAP12 and serves as an amplifier of cell activation in response to triggers such as LPS. TREM-1 activated cellular signalling molecules and pathways lead to generation of pro-inflammatory response such as expression and secretion of chemokines and cytokines including MCP-1, MIP-1a, interleukin 6 (IL-6), IL-8, and TNF. TREM-1 acts in a concerted manner with other signalling receptors such as TLRs and Nod-like receptors, leading to an amplification of the initial response. Expression of TREM-1 itself is upregulated in response to presence of LPS, microbial products, and cytokines released during activation response. A soluble form of TREM-1 (sTREM-1) is also detected in fluids from patients with infections.
Additional information: Clone REA213 displays negligible binding to Fc receptors.

Alternative names

TREM1

Detailed product information

Technical specifications

CloneREA213
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD354 (TREM-1)
Alternative names of antigenTREM1
Distribution of antigendendritic cells, macrophages, neutrophils
RRIDAB_2784150, AB_2657704, AB_2657705, AB_2657706, AB_2657707, AB_2657708, AB_2657709, AB_2657710, AB_2657711, AB_2657712, AB_2657713, AB_2657714, AB_2657715, AB_2657716, AB_2657717, AB_2657718, AB_2657719, AB_2784151

Resources for CD354 (TREM-1) Antibody, anti-human, REAfinity™

Certificates

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References for CD354 (TREM-1) Antibody, anti-human, REAfinity™

Publications

  1. Arts, R. J. W. et al. (2013) TREM-1: intracellular signaling pathways and interaction with pattern recognition receptors. J. Leukoc. Biol. 93: 209-215
  2. Ferat-Osorio, E. et al. (2008) The increased expression of TREM-1 on monocytes is associated with infectious and noninfectious inflammatory processes. J. Surg. Res. 150(1): 110-117

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