Clone:
REA1164
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
gp105-120, Mucosialin, My10

Extended validation for CD34 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1164
561++
581++
563++
8G12++
4H11++
QBEND/10-
AC136++
Cells were incubated with an excess of purified unconjugated CD34 (REA1164) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD34. Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies and with a suitable counterstaining. As a control, CD34 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD34 (REA1164). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD34 (REA1164). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD34 (REA1164). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD34 Antibody, anti-human, REAfinity™

Overview

Clone REA1164 recognizes a class III epitope of the CD34 antigen. This epitope is different than the one recognized by the clone used in the CD34 MicroBead Kits.
The CD34 antigen is a single-chain transmembrane glycoprotein, expressed on human hematopoitetic stem and progenitor cells, endothelial progenitor cells, vascular endothelial cells, embryonic fibroblasts, and some cells in fetal and adult nervous tissue. The antigen is absent on fully differentiated hematopoietic cells such as normal peripheral blood lymphocytes, monocytes, granulocytes, erythrocytes, and platelets. CD34 antibodies can be used for studies of hematopoiesis and non-hematopoiectic stem cells, phenotyping of hematopoietic stem cells, and studies on phenotyping of hematologic malignancies, endothelial cells, and endothelial progenitor cells (EPCs).
Additional information: Clone REA1164 displays negligible binding to Fc receptors.

Alternative names

gp105-120, Mucosialin, My10

Detailed product information

Technical specifications

CloneREA1164
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD34
Alternative names of antigengp105-120, Mucosialin, My10
Molecular mass of antigen [kDa]37
Distribution of antigenendothelial cells, stem cells, hematopoietic stem and progenitor cells, fetal neuronal progenitor cells, neural stem cells
Entrez Gene ID947
RRIDAB_2811339, AB_2811673, AB_2811668, AB_2811669, AB_2811674, AB_2811670, AB_2811345, AB_2811340, AB_2811346, AB_2811341, AB_2811338, AB_2811342, AB_2811337, AB_2811675, AB_2811671, AB_2811676, AB_2811672, AB_2811344, AB_2811343

Resources for CD34 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD34 Antibody, anti-human, REAfinity™

Publications

  1. Stull, R. A. et al. (2000) Simultaneous flow cytometric analyses of enhanced green and yellow fluorescent Cytometry 40: 126-134
  2. Metsuyanim, S. et al. (2009) Expression of stem cell markers in the human fetal kidney. PLoS One 4(68): e6709
  3. De Paepe, M. E. et al. (2011) Alveolar epithelial cell therapy with human cord blood-derived hematopoietic progenitor cells. Am. J. Pathol. 178(3): 1329-1339
  4. Bility, M. T. et al. (2012) Generation of a humanized mouse model with both human immune system and liver cells to model hepatitis C virus infection and liver immunopathogenesis. Nat. Protoc. 7(9): 1608-1617
  5. Vets, S. et al. (2012) Lens epithelium-derived growth factor/p75 qualifies as a target for HIV gene therapy in the NSG mouse model. Mol. Ther. 20(5): 908-917
  6. Liu, H. et al. (2013) Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells. Cell Death Dis 4: e857
  7. Lian, W. et al. (2014)
    Varying levels of 6-keto-prostaglandin F1α and thromboxane B2 in serum and endothelialization and hyperplasia in small-diameter grafts seeded with CD34
    +
    bone marrow cells in canines.
    Exp Ther Med. 7(5): 1123-1129

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