Clone:
REA302
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
ADGRE2, EMR2

Extended validation for CD312 (EMR2) Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD312 (EMR2) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD312 (EMR2)-PE (REA302, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD312 (EMR2) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD312 (EMR2)-PE (REA302, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD312 (EMR2) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD312 (EMR2)-PE (REA302, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD312 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD312-PE, clone (REA302). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD312 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD312-PE, clone (REA302). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD312 (EMR2) Antibody, anti-human, REAfinity™

Overview

Clone REA302 recognizes the human CD312 antigen, a multi-pass membrane protein also known as EGF-like module-containing mucin-like hormone receptor-like 2 (EMR2) which shares strikingly similar molecular characteristics with CD97. CD312 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins expressed predominantly by cells of the immune system. CD312 fails to interact with CD55, the cellular ligand for CD97, suggesting the possibility of different cellular ligands. CD312 interacts with chondroitin sulphate glycosaminoglycans in an isoform-specific manner. Expression of CD312 is up-regulated during differentiation and maturation of macrophages, and is conversely down-regulated during dendritic cell maturation. In monocytes and macrophages, CD312 can be specifically up-regulated by LPS and interleukin 10 (IL-10) via an IL-10–mediated pathway. In inflamed tissues, CD312 is detected in subpopulations of myeloid cells including macrophages and neutrophils. CD312 plays a role in the migration and adhesion of myeloid cells during cell differentiation, maturation, and activation.
Additional information: Clone REA302 displays negligible binding to Fc receptors.

Alternative names

ADGRE2, EMR2

Detailed product information

Technical specifications

CloneREA302
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD312 (EMR2)
Alternative names of antigenADGRE2, EMR2
Molecular mass of antigen [kDa]88
Distribution of antigenmyeloid leukemia cells, granulocytes, neutrophils
Entrez Gene ID30817
RRIDAB_2657349, AB_2657350, AB_2657351, AB_2657352, AB_2657353, AB_2657354, AB_2751831, AB_2819623, AB_2819619, AB_2751862

Resources for CD312 (EMR2) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD312 (EMR2) Antibody, anti-human, REAfinity™

Publications

  1. Kwakkenbos, M. J. et al. (2002) The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells. J. Leukoc. Biol. 71(5): 854-862
  2. Chang, G. W. et al. (2007) CD312, the human adhesion-GPCR EMR2, is differentially expressed during differentiation, maturation, and activation of myeloid cells. Biochem. Biophys. Res. Commun. 353(1): 133-138
  3. Yona, S. et al. (2008) Ligation of the adhesion-GPCR EMR2 regulates human neutrophil function. FASEB J. 22(3): 741-751

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