Clone:
REAL123
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC, MICS, IHC, IF
Alternative names:
PECAM1, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, EndoCAM

Extended validation for CD31 Antibody, anti-human,
REAlease
®

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD31 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD31-PE (REAL123, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD31 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD31-PE (REAL123, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD31 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD31-PE (REAL123, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of human CD31 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD31-PE, clone (REAL123). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of human CD31 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD31-PE, clone (REAL123). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD31 Antibody, anti-human,
REAlease
®

Overview

Clone REAL123 is an antibody fragment derived from the full CD31 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL123 recognizes the human CD31 antigen, also known as PECAM-1. CD31 is a member of the immunoglobulin superfamily and is involved in leukocyte transmigration, angiogenesis, and integrin activation. CD31 is a 120–140 kDa single-chain transmembrane glycoprotein expressed on endothelial cells, monocytes, platelets, granulocytes, and several lymphocyte subsets. During maturation of CD4+ T cells, the expression of CD31 changes: CD4
+
recent thymic emigrants (RTEs) express CD31 in contrast to central naive T cells. CD31 is highly expressed on endothelial cells, especially at their juncture.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

PECAM1, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, EndoCAM

Detailed product information

Technical specifications

CloneREAL123
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD31
Alternative names of antigenPECAM1, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, EndoCAM
Distribution of antigenendothelial cells, monocytes, platelets, granulocytes, lymphocytes
RRIDAB_2751066, AB_2784129, AB_2751090

Resources for CD31 Antibody, anti-human,
REAlease
®

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD31 Antibody, anti-human,
REAlease
®

Publications

  1. Newman, P. J. et al. (1990) PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247(4947): 1219-1222
  2. Stockinger, H. et al. (1990) Molecular characterization and functional analysis of the leukocyte surface protein CD31. J. Immunol. 145(11): 3889-3897
  3. Kimmig, S. et al. (2002) Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood. J. Exp. Med. 195: 789-794

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REAlease
®

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