Clone:
AC128
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ, mouse IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
PECAM1, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, EndoCAM

Extended validation for CD31 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with AC128
MBC.78:2-
L133.1-
WM59-
MEM-05++
1F11-
M89D3-
REA1028++
Cells were incubated with an excess of purified unconjugated CD31 (AC128) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD31 Antibody, anti-human

Overview

CD31, a 120–140 kDa single-chain transmembrane glycoprotein, is present on virtually all monocytes, platelets, and granulocytes. CD31 is also expressed by lymphocyte subsets. During maturation of CD4
+
T cells, the expression of CD31 changes: CD4
+
recent thymic emigrants (RTEs) express CD31 in contrast to central naive T cells. CD31 is highly expressed on endothelial cells, especially at their juncture, and is also known EndoCAM.

Alternative names

PECAM1, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, EndoCAM

Detailed product information

Technical specifications

CloneAC128
Clonalitymonoclonal
Isotypemouse IgG1κ, mouse IgG1
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
AntigenCD31
Alternative names of antigenPECAM1, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, EndoCAM
Molecular mass of antigen [kDa]80
Distribution of antigenendothelial cells, fibroblasts, granulocytes, macrophages, monocytes, NK cells, osteoclasts, platelets, T cells, leukocytes, lymphocytes, basophils, neutrophils, plasma cells, granulocytes, endothelial cells, fibroblasts, leukocytes, lymphocytes, macrophages, monocytes, NK cells, osteoclasts, platelets, T cells, basophils, neutrophils, plasma cells
Entrez Gene ID5175
RRIDAB_2733637, AB_2733806, AB_2733807, AB_2784125, AB_2784124, AB_2784127, AB_2784126, AB_2751958, AB_2751911, AB_2751956, AB_2751909, AB_2751957, AB_2751910, AB_2660571, AB_2733636

Resources for CD31 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

Reviews for CD31 Antibody, anti-human

Great CD31 Antibody from Miltenyi Biotec

  • 1
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CD31-FITC, human (130-117-390)

This antibody gives specific signal on frozen-tissue sections with little background. Publishable results!

References for CD31 Antibody, anti-human

Publications

  1. Kimmig, S. et al. (2002) Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood. J. Exp. Med. 195: 789-794

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