Clone:
MIH12
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC
Alternative names:
ICOSLG, B7-H2, B7H2, B7RP-1, GL50, ICOSL, LICOS

Extended validation for CD275 (B7-H2) Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with MIH12
REA991++
2D3-
9F.8A4+
136726++
Cells were incubated with an excess of purified unconjugated CD275 (B7-H2) (MIH12) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD275 (B7-H2) Antibody, anti-human

Overview

Clone MIH12 recognizes CD275 (B7-H2, ICOSL, GL50), a member of the B7 family of costimulatory molecules involved in regulating T cell activation and effector functions. It belongs to Ig superfamily and has a predicted molecular weight of 40 kDa. CD275 is a ligand of inducible costimulator (ICOS) expressed on activated T cells. ICOS mediated costimulation promotes T-helper cell differentiation via Tʜ2 cytokine production such as IL-10 and IL-4. Human CD275 is expressed on B cells, dendritic cells, endothelial cells, renal tubular epithelial cells, activated monocytes, and human airway epithelial cells.

Alternative names

ICOSLG, B7-H2, B7H2, B7RP-1, GL50, ICOSL, LICOS

Detailed product information

Technical specifications

CloneMIH12
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD275 (B7-H2)
Alternative names of antigenICOSLG, B7-H2, B7H2, B7RP-1, GL50, ICOSL, LICOS
Molecular mass of antigen [kDa]31
Distribution of antigenB cells, dendritic cells, endothelial cells, epithelial cells, monocytes
Entrez Gene ID23308
RRIDAB_2656888, AB_2656889, AB_2656890, AB_2656891, AB_2656892, AB_2656893, AB_2656894, AB_2656895, AB_2656896, AB_2656887

Resources for CD275 (B7-H2) Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD275 (B7-H2) Antibody, anti-human

Publications

  1. Wang, S. et al. (2000) Costimulation of T cells by B7-H2, a B7-like molecule that binds ICOS. Blood 96(8): 2808-2813
  2. Qian, X. et al. (2006) The ICOS-ligand B7-H2, expessed on human type II alveolar epithelial cells, plays a role in the pulmonary host defense system. Eur. J. Immunol. 36(4): 906-918
  3. Kurosawa, S. et al. (2003) Expression of the costimulatory molecule B7-H2 (inducible costimulator ligand) by human airway epithelial cells. Am. J. Respir. Cell Mol. Biol. 28: 563-573

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