Clone:
REA574
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MC
Alternative names:
CCR3, CC-CKR-3, CKR3, CMKBR3

Extended validation for CD193 (CCR3) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA574
5E8.4++
Cells were incubated with an excess of purified unconjugated CD193 (CCR3) (REA574) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD193 (CCR3). Human peripheral blood mononuclear cells (PBMCs) were stained with CD193 (CCR3) antibodies and with a suitable counterstaining. As a control, CD193 (CCR3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD193 (CCR3). Human peripheral blood mononuclear cells (PBMCs) were stained with CD193 (CCR3) antibodies and with a suitable counterstaining. As a control, CD193 (CCR3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD193 (CCR3). Human peripheral blood mononuclear cells (PBMCs) were stained with CD193 (CCR3) antibodies and with a suitable counterstaining. As a control, CD193 (CCR3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD193 (CCR3). Human peripheral blood mononuclear cells (PBMCs) were stained with CD193 (CCR3) antibodies and with a suitable counterstaining. As a control, CD193 (CCR3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD193 (CCR3). Human peripheral blood mononuclear cells (PBMCs) were stained with CD193 (CCR3) antibodies and with a suitable counterstaining. As a control, CD193 (CCR3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD193 (CCR3) (REA574). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD193 (CCR3) (REA574). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD193 (CCR3) (REA574). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD193 (CCR3) Antibody, anti-human, REAfinity™

Overview

Clone REA547 recognizes the human CD193 antigen, a seven-transmembrane domain G protein–coupled receptor, also known as CCR3. CD193 binds a variety of chemokines, including eotaxin (CCL11), eotaxin-2 (CCL24), eotaxin-3 (CCL26), RANTES (CCL5), MCP-4 (CCL13), leukotactin (CCL15), and MEC (CCL28). CD193 is expressed by eosinophils, basophils, mast cells, platelets, CD34
+
hematopoietic progenitor cells, and keratinocytes, as well as by some T cell subsets, in particular Tʜ2 cells. CD193 regulates cell migration and activation and is a co-receptor for HIV.
Additional information: Clone REA547 displays negligible binding to Fc receptors.

Alternative names

CCR3, CC-CKR-3, CKR3, CMKBR3

Detailed product information

Technical specifications

CloneREA574
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD193 (CCR3)
Alternative names of antigenCCR3, CC-CKR-3, CKR3, CMKBR3
Molecular mass of antigen [kDa]41
Distribution of antigenmast cells, platelets, T cells, eosinophils, basophils
Entrez Gene ID1232
RRIDAB_2727580, AB_2751790, AB_2751730, AB_2819481, AB_2819469, AB_2801901, AB_2655890, AB_2655891, AB_2655894, AB_2655895, AB_2727644

Resources for CD193 (CCR3) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD193 (CCR3) Antibody, anti-human, REAfinity™

Publications

  1. Ponath, P. D. et al. (1996) Molecular cloning and characterization of a human eotaxin receptor expressed selectively on eosinophils. J. Exp. Med. 183(6): 2437-2448
  2. Combadiere, C. et al. (1995) Cloning and functional expression of a human eosinophil CC chemokine receptor. J. Biol. Chem. 270(28): 16491-16494
  3. Xiao, L. et al. (1999) Partial resistance to infection by R5X4 primary HIV type 1 isolates in an exposed-uninfected individual homozygous for CCR5 32-base pair deletion. AIDS Res Hum Retroviruses 15(13): 1201-1208

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