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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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CD19 + cells were isolated from human PBMCs using CD19 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-PE and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Unseparated fraction | After separation |
Figure 1CD19 + cells were isolated from human PBMCs using CD19 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-PE and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. | Figure 1CD19 + cells were isolated from human PBMCs using CD19 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-PE and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
The primary goal of our research was to obtain highly pure CD19+ B cells, as well as other immune cell types, from human peripheral blood mononuclear cells. The cells were required to be highly pure since downstream analysis involved microarray analysis of gene expression. This product was selected over competing products in part due to its ease of use and the previously established and protocols provided by the manufacturer.
We are interested in antibody responses that protect against HIV infection, and engineering B cells to express bNAb. We use a humanized mouse model consisting of activated PBMC injected into NSG mouse spleens. We have been setting up experiments where we transduce B cells with lentiviral vectors expressing different bNAb vectors and injected them into the mice to investigate their protective capacity. We purify B cells using these beads.
CD19 + cells were isolated from human PBMCs using CD19 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-PE and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Unseparated fraction | After separation |
Figure 1CD19 + cells were isolated from human PBMCs using CD19 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-PE and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. | Figure 1CD19 + cells were isolated from human PBMCs using CD19 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-PE and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
The primary goal of our research was to obtain highly pure CD19+ B cells, as well as other immune cell types, from human peripheral blood mononuclear cells. The cells were required to be highly pure since downstream analysis involved microarray analysis of gene expression. This product was selected over competing products in part due to its ease of use and the previously established and protocols provided by the manufacturer.
We are interested in antibody responses that protect against HIV infection, and engineering B cells to express bNAb. We use a humanized mouse model consisting of activated PBMC injected into NSG mouse spleens. We have been setting up experiments where we transduce B cells with lentiviral vectors expressing different bNAb vectors and injected them into the mice to investigate their protective capacity. We purify B cells using these beads.
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