Clone:
REA1201
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
SHPS-1, SIRPα, P84, Sirpa, Bit, Myd1, Ptpns1, Shps1, Sirp

Extended validation for CD172a (SIRPα) Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1201
P84++
772734-
Cells were incubated with an excess of purified unconjugated CD172a (SIRPα) (REA1201) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD172a (SIRPα) (REA1201). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD172a (SIRPα) (REA1201). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD172a (SIRPα) (REA1201). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD172a (SIRPα) Antibody, anti-mouse, REAfinity™

Overview

Clone REA1201 recognizes the mouse antigen CD172a, a 75–110 kDa glycosylated receptor-like protein, which is also known as SIRPα or SHPS-1. CD172a promotes the attachment and neurite outgrowth of cultured murine cerebellar cells. It binds to its ligand CD47, which inhibits cytokine production by mature dendritic cells (DCs) and avoids maturation of immature DCs. CD172a plays a role in the negative regulation of receptor tyrosine kinase–coupled cellular responses induced by cell adhesion, growth factors, or insulin and mediates negative regulation of phagocytosis, mast cell, and DC activation. CD172a is strongly expressed by neurons and myeloid cells, such as monocytes, macrophages, granulocytes, and DCs in several tissues, e.g., cerebral cortex, brain, spinal cord, cerebellum, and spleen. CD172a is not expressed on oligodendrocytes or GFAP-positive astrocytes.
Additional information: Clone REA1201 displays negligible binding to Fc receptors.

Alternative names

SHPS-1, SIRPα, P84, Sirpa, Bit, Myd1, Ptpns1, Shps1, Sirp

Detailed product information

Technical specifications

CloneREA1201
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD172a (SIRPα)
Alternative names of antigenSHPS-1, SIRPα, P84, Sirpa, Bit, Myd1, Ptpns1, Shps1, Sirp
Molecular mass of antigen [kDa]53
Distribution of antigenneurons, myeloid cells, macrophages, monocytes, granulocytes, dendritic cells, spleen, brain, spinal cord
Entrez Gene ID19261
RRIDAB_2802005, AB_2802006, AB_2802007, AB_2802002, AB_2802003, AB_2802004, AB_2802001

Resources for CD172a (SIRPα) Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD172a (SIRPα) Antibody, anti-mouse, REAfinity™

Publications

  1. Chuang, W. et al. (1990) Central nervous system antigen P84 can serve as a substrate for neurite outgrowth. Dev. Biol. 137(2): 219-232
  2. Comu, S. et al. (1997) The murine P84 neural adhesion molecule is SHPS-1, a member of the phosphatase-binding protein family. J. Neurosci. 17(22): 8702-8710

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