Clone:
191B8
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2aκ
Applications:
FC, MICS, IF, IHC
Alternative names:
KLRB1, CLEC5B, NKR, NKR-P1, NKR-P1A, NKRP1A, HNKR-P1a

Extended validation for CD161 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 191B8
702228++
REA631++
DX12-
HP-3G10++
Cells were incubated with an excess of purified unconjugated CD161 (191B8) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD161. Human peripheral blood mononuclear cells (PBMCs) were stained with CD161 antibodies and with a suitable counterstaining. As a control, CD161 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD161. Human peripheral blood mononuclear cells (PBMCs) were stained with CD161 antibodies and with a suitable counterstaining. As a control, CD161 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD161. Human peripheral blood mononuclear cells (PBMCs) were stained with CD161 antibodies and with a suitable counterstaining. As a control, CD161 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD161. Human peripheral blood mononuclear cells (PBMCs) were stained with CD161 antibodies and with a suitable counterstaining. As a control, CD161 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD161. Human peripheral blood mononuclear cells (PBMCs) were stained with CD161 antibodies and with a suitable counterstaining. As a control, CD161 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD161 (191B8). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD161 (191B8). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD161 (191B8). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD161 Antibody, anti-human

Overview

Clone 191B8 recognizes CD161, a C-type lectin membrane glycoprotein. CD161, also known as NKR-P1A, is expressed as an 80 kDa disulfide-linked homodimer and is found on most NK cells and subsets of T cells. In peripheral blood, CD161 is preferentially expressed on T cells of memory phenotype, but it can also be found on subsets of thymocytes and fetal liver T cells.
CD161 has been implicated in triggering NK-mediated cytotoxicity, contributing to target cell recognition by NK cells.

Alternative names

KLRB1, CLEC5B, NKR, NKR-P1, NKR-P1A, NKRP1A, HNKR-P1a

Detailed product information

Technical specifications

Clone191B8
Clonalitymonoclonal
Isotypemouse IgG2aκ
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD161
Alternative names of antigenKLRB1, CLEC5B, NKR, NKR-P1, NKR-P1A, NKRP1A, HNKR-P1a
Molecular mass of antigen [kDa]25
Distribution of antigenNK cells, T cells, thymocytes, liver
Entrez Gene ID3820
RRIDAB_2733625, AB_2733771, AB_2733772, AB_2733345, AB_2733346, AB_2751200, AB_2751134, AB_2733504, AB_2733505, AB_871628, AB_2733624

Resources for CD161 Antibody, anti-human

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for CD161 Antibody, anti-human

Publications

  1. Lanier, L. L. et al. (1994) Human NKR-P1A. A disulfide-linked homodimer of the C-type lectin superfamily expressed by a subset of NK and T lymphocytes. J. Immunol. 153: 2417-2428
  2. Poggi, A. et al. (2002)
    Transendothelial migratory pathways of Vδ1
    +
    TCRγδ
    +
    and Vδ2
    +
    TCRγδ
    +
    T lymphocytes from healthy donors and multiple sclerosis patients: involvement of phosphatidylinositol 3 kinase and calcium calmodulin-dependent kinase II.
    J. Immunol. 168: 6071-6077
  3. Pozo, D. et al. (2006) CD161 (human NKRP1A) signaling in NK cells involves the activation of acid sphingomyelinase. J. Immunol. 176: 2397-2406
  4. Takahashi, T. et al. (2006) Expression of CD161 (NKR-P1A) defines subsets of human CD4 and CD8 T cells with different functional activities.​ J. Immunol. 176: 211-216

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