Clone:
REA1010
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MC
Alternative names:
KIR2DL1, KIR-K64, KIR221, NKAT, NKAT-1, p58.1, KIR2DS1

Extended validation for CD158a/h (KIR2DL1/DS1) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1010
11PB6++
HP-MA4++
EB6B++
Cells were incubated with an excess of purified unconjugated CD158a/h (KIR2DL1/DS1) (REA1010) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD158a/h (KIR2DL1/DS1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158a/h (KIR2DL1/DS1) antibodies and with a suitable counterstaining. As a control, CD158a/h (KIR2DL1/DS1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158a/h (KIR2DL1/DS1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158a/h (KIR2DL1/DS1) antibodies and with a suitable counterstaining. As a control, CD158a/h (KIR2DL1/DS1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158a/h (KIR2DL1/DS1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158a/h (KIR2DL1/DS1) antibodies and with a suitable counterstaining. As a control, CD158a/h (KIR2DL1/DS1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158a/h (KIR2DL1/DS1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158a/h (KIR2DL1/DS1) antibodies and with a suitable counterstaining. As a control, CD158a/h (KIR2DL1/DS1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD158a/h (KIR2DL1/DS1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158a/h (KIR2DL1/DS1) antibodies and with a suitable counterstaining. As a control, CD158a/h (KIR2DL1/DS1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD158a/h (KIR2DL1/DS1) (REA1010). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD158a/h (KIR2DL1/DS1) (REA1010). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD158a/h (KIR2DL1/DS1) (REA1010). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD158a/h (KIR2DL1/DS1) Antibody, anti-human, REAfinity™

Overview

Clone REA1010 recognizes the human CD158a (KIR2DL1) and CD158h (KIR2DS1) antigens, two members of the killer immunoglobulin-like receptor (KIR) family expressed on CD56
dim
CD16
+
natural killer (NK) cells and CD8
+
T cells. CD158a (KIR2DL1) is involved in the transduction of an inhibitory signal whereas CD158h (KIR2DS1) is an activating receptor. The ligands of CD158a (KIR2DL1) are HLA-Cw4 and related molecules.
Additional information: Clone REA1010 displays negligible binding to Fc receptors.

Alternative names

KIR2DL1, KIR-K64, KIR221, NKAT, NKAT-1, p58.1, KIR2DS1

Detailed product information

Technical specifications

CloneREA1010
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD158a/h (KIR2DL1/DS1)
Alternative names of antigenKIR2DL1, KIR-K64, KIR221, NKAT, NKAT-1, p58.1, KIR2DS1
Molecular mass of antigen [kDa]36
Distribution of antigenNK cells, T cells, CD8+ T cells
Entrez Gene ID3802
RRIDAB_2727794, AB_2727722, AB_2727795, AB_2727723, AB_2727796, AB_2727724, AB_2727725, AB_2727726, AB_2727797, AB_2727727, AB_2801922

Resources for CD158a/h (KIR2DL1/DS1) Antibody, anti-human, REAfinity™

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD158a/h (KIR2DL1/DS1) Antibody, anti-human, REAfinity™

Publications

  1. Falco, M. et al. (2010) Combined genotypic and phenotypic killer cell Ig-like receptor analyses reveal KIR2DL3 alleles displaying unexpected monoclonal antibody reactivity: identification of the amino acid residues critical for staining. J. Immunol. 185: 433-441

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