Clone:
BNI3
Type of antibody:
Primary antibodies, Functional-grade antibodies
Isotype:
mouse IgG2aκ
Applications:
ICFC, FA
Alternative names:
CTLA4, ALPS5, CD, CELIAC3, CTLA-4, GRD4, GSE, IDDM12

Extended validation for CD152 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with BNI3
L3D10++
14D3-
REA1003++
Cells were incubated with an excess of purified unconjugated CD152 (BNI3) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD152. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with CD152 antibodies. As a control, CD152 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD152. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with CD152 antibodies. As a control, CD152 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD152. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with CD152 antibodies. As a control, CD152 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD152. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with CD152 antibodies. As a control, CD152 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD152. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with CD152 antibodies. As a control, CD152 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD152 Antibody, anti-human

Overview

The monoclonal antibody BNI3 reacts with human CD152, an integral membrane protein of the immunoglobin superfamily which is also called CTLA-4. CD152 is a negative regulator of T cell activation. It is transiently expressed on activated CD28
+
T cells and is a ligand for CD80 and CD86. CD4
+
FoxP3
+
regulatory T cells are reported to constitutively express CD152. CD152 may also be detected on B cells when cultured with activated T cells.

Alternative names

CTLA4, ALPS5, CD, CELIAC3, CTLA-4, GRD4, GSE, IDDM12

Detailed product information

Technical specifications

CloneBNI3
Clonalitymonoclonal
Isotypemouse IgG2aκ
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies, Functional-grade antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
,
chimpanzee (
Pan troglodytes
)
,
olive baboon (
Papio anubis
)
AntigenCD152
Alternative names of antigenCTLA4, ALPS5, CD, CELIAC3, CTLA-4, GRD4, GSE, IDDM12
Molecular mass of antigen [kDa]21
Distribution of antigenB cells, T cells
Entrez Gene ID1493
RRIDAB_2733767, AB_2857662, AB_2857639, AB_2655248, AB_2733766

Resources for CD152 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD152 Antibody, anti-human

Publications

  1. Kuiper, H. M. et al. (1995) Activated T cells can induce high levels of CTLA-4 expression on B cells. J. Immunol. 155(4): 1776-1783
  2. Castan, J. et al. (1997) Accumulation of CTLA-4 expressing T lymphocytes in the germinal centres of human lymphoid tissues. Immunology 90(2): 265-271
  3. Sansom, D. M. and Walker, L. S. (2006) The role of CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4) in regulatory T-cell biology. Immunol. Rev. 212: 131-148
  4. Schlotmann, T. et al. (2000) CD alphabeta T lymphocytes express high levels of the T lymphocyte antigen CTLA-4 (CD152) in acute malaria. J. Infect. Dis. 182(1): 367-370

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