Clone:
REA637
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
PDGF-R-alpha, PDGFR-alpha, PDGFR-α

Extended validation for CD140a Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA637
APA5++
Cells were incubated with an excess of purified unconjugated CD140a (REA637) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD140a. 3T3-A31 cells were stained with CD140a antibodies and plotted against the side scatter. As a control, CD140a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD140a. 3T3-A31 cells were stained with CD140a antibodies and plotted against the side scatter. As a control, CD140a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD140a. 3T3-A31 cells were stained with CD140a antibodies and plotted against the side scatter. As a control, CD140a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD140a. 3T3-A31 cells were stained with CD140a antibodies and plotted against the side scatter. As a control, CD140a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD140a. 3T3-A31 cells were stained with CD140a antibodies and plotted against the side scatter. As a control, CD140a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD140a (REA637). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD140a (REA637). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD140a (REA637). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD140a Antibody, anti-mouse, REAfinity™

Overview

Clone REA637 recognizes the mouse CD140a antigen, a single-pass type I membrane protein, also known as platelet-derived growth factor receptor alpha (PDGFRα). CD140a is a receptor tyrosine kinase that forms homodimers or heterodimers with CD140b (PDGFRβ). Receptor dimerization and transphosphorylation at tyrosine residues activates the intracellular kinase activity. CD140a is broadly expressed in embryonic tissue, mostly in cells of mesodermal origin in adult tissue, and embryonic stem cell–derived cardiomyogenic cells. Expression of CD140a is associated with various malignancies.
Additional information: Clone REA637 displays negligible binding to Fc receptors.

Alternative names

PDGF-R-alpha, PDGFR-alpha, PDGFR-α

Detailed product information

Technical specifications

CloneREA637
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD140a
Alternative names of antigenPDGF-R-alpha, PDGFR-alpha, PDGFR-α
Molecular mass of antigen [kDa]120
Distribution of antigenepithelial cells, fibroblasts, monocytes, osteoblasts, red blood cells, mesenchymal stem cells, bone marrow
Entrez Gene ID18595
RRIDAB_2801977, AB_2857824, AB_2655068, AB_2655069, AB_2655074, AB_2655075, AB_2655078, AB_2655079, AB_2655080, AB_2655081, AB_2819411

Resources for CD140a Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for CD140a Antibody, anti-mouse, REAfinity™

Publications

  1. Takakura, N. et al. (1997) PDGFRα expression during mouse embryogenesis: immunolocalization analyzed by whole-mount immunohistostaining using the monoclonal antibody anti-mouse PDGFR alpha antibody APA5. J. Histochem. Cytochem. 45(6): 883-893
  2. Ntokou, A. et al. (2015) Characterization of the platelet-derived growth factor receptor α-positive cell lineage during murine late lung development. Am. J. Physiol. Lung Cell Mol. Physiol. 309(9): L942-948
  3. Zhou, Q. et al. (2015) Cardiac telocytes are double positive for CD34/PDGFR‑α. J. Cell. Mol. Med. 19(8): 2036-2042

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