Clone:
ACT35
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC
Alternative names:
TNFRSF4, IMD16, OX-40, Txgp1l, ACT35

Extended validation for CD134 (OX40) Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with ACT35
Ber-ACT35++
L106-
443318 rIgG2a++
REA621++
Cells were incubated with an excess of purified unconjugated CD134 (OX40) (ACT35) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD134 (OX40). Human peripheral blood mononuclear cells (PBMCs) stimulated with CytoStim™ for 16h were stained with CD134 (OX40) antibodies and with a suitable counterstaining. As a control, CD134 (OX40) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD134 (OX40). Human peripheral blood mononuclear cells (PBMCs) stimulated with CytoStim™ for 16h were stained with CD134 (OX40) antibodies and with a suitable counterstaining. As a control, CD134 (OX40) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD134 (OX40). Human peripheral blood mononuclear cells (PBMCs) stimulated with CytoStim™ for 16h were stained with CD134 (OX40) antibodies and with a suitable counterstaining. As a control, CD134 (OX40) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD134 (OX40). Human peripheral blood mononuclear cells (PBMCs) stimulated with CytoStim™ for 16h were stained with CD134 (OX40) antibodies and with a suitable counterstaining. As a control, CD134 (OX40) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD134 (OX40). Human peripheral blood mononuclear cells (PBMCs) stimulated with CytoStim™ for 16h were stained with CD134 (OX40) antibodies and with a suitable counterstaining. As a control, CD134 (OX40) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD134 (OX40) (ACT35). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD134 (OX40) (ACT35). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD134 (OX40) (ACT35). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD134 (OX40) Antibody, anti-human

Overview

Clone ACT35 recognizes the CD134 (OX40) antigen, a member of the tumor necrosis factor/nerve growth factor receptor (TNFR/NGFR) family. CD134 is a 50 kDa type I membrane glycoprotein expressed by activated T lymphocytes. The interaction of CD134 with OX40L has been implicated in T cell–dependent humoral response, regulation of primary T cell expansion, survival of T cells, size of the memory T cell pool, and regulation of tolerance in the CD4
+
T cell compartment.

Alternative names

TNFRSF4, IMD16, OX-40, Txgp1l, ACT35

Detailed product information

Technical specifications

CloneACT35
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD134 (OX40)
Alternative names of antigenTNFRSF4, IMD16, OX-40, Txgp1l, ACT35
Molecular mass of antigen [kDa]27
Distribution of antigenB cells, endothelial cells, fibroblasts, T cells, lymphocytes
Entrez Gene ID7293
RRIDAB_2727566, AB_2661313, AB_2661314, AB_10828448, AB_2661315, AB_2661316, AB_10830554, AB_2727640

Resources for CD134 (OX40) Antibody, anti-human

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD134 (OX40) Antibody, anti-human

Publications

  1. Zaunders, J. J. et al. (2009)
    High levels of human antigen-specific CD4
    +
    T cells in peripheral blood revealed by stimulated coexpression of CD25 and CD134 (OX40).
    J. Immunol. 183: 2827-2836
  2. Croft, M. et al. (2009) The significance of OX40 and OX40L to T-cell biology and immune disease. Immunol. Rev. 229(1): 173-191

Related products for
CD134 (OX40) Antibody, anti-human

4 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?