Clone:
REA193
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
CSF2RB, AIC2B, Bc, CDw131, Csf2rb1, Csfgmrb, IL3R, IL3RB, Il3rb1, IL5RB

Extended validation for CD131 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA193
JORO50++
Cells were incubated with an excess of purified unconjugated CD131 (REA193) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD131. Bone marrow cells from C57BL/6 mice were stained with CD131 antibodies and with a suitable counterstaining. As a control, CD131 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD131. Bone marrow cells from C57BL/6 mice were stained with CD131 antibodies and with a suitable counterstaining. As a control, CD131 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD131. Bone marrow cells from C57BL/6 mice were stained with CD131 antibodies and with a suitable counterstaining. As a control, CD131 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD131. Bone marrow cells from C57BL/6 mice were stained with CD131 antibodies and with a suitable counterstaining. As a control, CD131 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD131 (REA193). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD131 (REA193). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD131 (REA193). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD131 Antibody, anti-mouse, REAfinity™

Overview

Clone REA193 recognizes CD131, the IL-3 receptor β subunit. CD131 is a single-pass type I transmembrane protein, belongs to the cytokine receptor superfamily, and is shared by IL-3, IL-5, and GM-CSF receptors. The receptor-specific α subunits is mainly involved in ligand binding and the common β subunit, CD131, is important for transducing the signal initiated by binding of the ligands to respective receptors.
Additional information: Clone REA193 displays negligible binding to Fc receptors.

Alternative names

CSF2RB, AIC2B, Bc, CDw131, Csf2rb1, Csfgmrb, IL3R, IL3RB, Il3rb1, IL5RB

Detailed product information

Technical specifications

CloneREA193
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD131
Alternative names of antigenCSF2RB, AIC2B, Bc, CDw131, Csf2rb1, Csfgmrb, IL3R, IL3RB, Il3rb1, IL5RB
Molecular mass of antigen [kDa]97
Distribution of antigengranulocytes, monocytes, eosinophils, basophils
Entrez Gene ID12983
RRIDAB_2654863, AB_2654864, AB_2654865, AB_2654866, AB_2654867, AB_2654868, AB_2733762

Resources for CD131 Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD131 Antibody, anti-mouse, REAfinity™

Publications

  1. Goodall, G. J. et al. (1993) A model for the interaction of the GM-CSF, IL-3 and IL-5 receptors with their ligands. Growth Factors 8(2): 87-97
  2. Guthridge, M. A. et al. (1998) Mechanism of activation of the GM-CSF, IL-3, and IL-5 family of receptors. Stem Cells 16(5): 301-313

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